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Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells.

Anisimov, Sergey; Christophersen, Nicolaj LU ; Correia, Ana S; Hall, Vanessa; Sandelin, Ingrid; Li, Jia-Yi LU and Brundin, Patrik LU (2011) In Cellular & Molecular Biology Letters 16(1). p.79-88
Abstract
The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual... (More)
The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cellular & Molecular Biology Letters
volume
16
issue
1
pages
79 - 88
publisher
Versita
external identifiers
  • wos:000288814600006
  • pmid:21161417
  • scopus:79955547570
ISSN
1689-1392
DOI
10.2478/s11658-010-0039-8
language
English
LU publication?
yes
id
46d027bf-87a1-46b5-9a7e-ca2e25f53a62 (old id 1756276)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21161417?dopt=Abstract
date added to LUP
2011-01-03 15:45:21
date last changed
2017-11-19 03:50:58
@article{46d027bf-87a1-46b5-9a7e-ca2e25f53a62,
  abstract     = {The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.},
  author       = {Anisimov, Sergey and Christophersen, Nicolaj and Correia, Ana S and Hall, Vanessa and Sandelin, Ingrid and Li, Jia-Yi and Brundin, Patrik},
  issn         = {1689-1392},
  language     = {eng},
  number       = {1},
  pages        = {79--88},
  publisher    = {Versita},
  series       = {Cellular & Molecular Biology Letters},
  title        = {Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells.},
  url          = {http://dx.doi.org/10.2478/s11658-010-0039-8},
  volume       = {16},
  year         = {2011},
}