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Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning

Wåhlander, Åsa LU ; Arrigoni, Giorgio LU ; Wårell, Kristofer LU ; Levander, Fredrik LU orcid ; Palmgren, Ronnie ; Maloisel, Jean-Luc ; Busson, Philippe and James, Peter LU orcid (2007) In Journal of Proteome Research 6(3). p.1101-1113
Abstract
We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
precursor-ion, reagents, mass spectrometry, isotope labeling, HPLC, parent-ion
in
Journal of Proteome Research
volume
6
issue
3
pages
1101 - 1113
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000244638400018
  • scopus:33947606962
ISSN
1535-3893
DOI
10.1021/pr0604312
language
English
LU publication?
yes
id
17594dd4-1021-4cd4-9409-d9a2919bf26a (old id 672594)
date added to LUP
2016-04-01 12:32:07
date last changed
2025-10-14 10:56:37
@article{17594dd4-1021-4cd4-9409-d9a2919bf26a,
  abstract     = {{We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.}},
  author       = {{Wåhlander, Åsa and Arrigoni, Giorgio and Wårell, Kristofer and Levander, Fredrik and Palmgren, Ronnie and Maloisel, Jean-Luc and Busson, Philippe and James, Peter}},
  issn         = {{1535-3893}},
  keywords     = {{precursor-ion; reagents; mass spectrometry; isotope labeling; HPLC; parent-ion}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{1101--1113}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Proteome Research}},
  title        = {{Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning}},
  url          = {{http://dx.doi.org/10.1021/pr0604312}},
  doi          = {{10.1021/pr0604312}},
  volume       = {{6}},
  year         = {{2007}},
}