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Surrogate antigens as targets for proteome-wide binder selection

Gustavsson, Elin LU ; Ek, Sara LU ; Steen, Johanna; Nordström, Malin LU ; Älgenäs, Cajsa; Uhlén, Mathias; Wingren, Christer LU ; Ottosson, Jenny; Hober, Sophia and Borrebaeck, Carl LU (2011) In New Biotechnology 28. p.302-311
Abstract
In the past decade, many initiatives were taken for the development of antibodies for proteome-wide studies, as well as characterisation and validation of clinically relevant disease biomarkers. Phage display offers many advantages compared to antibody generation by immunisation because it is an unlimited resource of affinity reagents without batch-to-batch variation and is also amendable for high throughput in contrast to conventional hybridoma technology. One of the major bottlenecks to proteome-wide binder selection is the limited supply of suitable target antigens representative of the human proteome. Here, we provide proof of principle of using easily accessible, cancer-associated protein epitope signature tags (PrESTs), routinely... (More)
In the past decade, many initiatives were taken for the development of antibodies for proteome-wide studies, as well as characterisation and validation of clinically relevant disease biomarkers. Phage display offers many advantages compared to antibody generation by immunisation because it is an unlimited resource of affinity reagents without batch-to-batch variation and is also amendable for high throughput in contrast to conventional hybridoma technology. One of the major bottlenecks to proteome-wide binder selection is the limited supply of suitable target antigens representative of the human proteome. Here, we provide proof of principle of using easily accessible, cancer-associated protein epitope signature tags (PrESTs), routinely generated within the Human Protein Atlas project, as surrogate antigens for full-length proteins in phage selections for the retrieval of target-specific binders. These binders were subsequently tested in western blot, immunohistochemistry and protein microarray application to demonstrate their functionality. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
New Biotechnology
volume
28
pages
302 - 311
publisher
Elsevier
external identifiers
  • wos:000292717500002
  • scopus:79958056952
ISSN
1876-4347
DOI
10.1016/j.nbt.2010.12.005
language
English
LU publication?
yes
id
7cba536f-9d37-45aa-8f78-67e96ccb56af (old id 1761106)
date added to LUP
2011-01-11 11:46:34
date last changed
2017-04-09 03:11:43
@article{7cba536f-9d37-45aa-8f78-67e96ccb56af,
  abstract     = {In the past decade, many initiatives were taken for the development of antibodies for proteome-wide studies, as well as characterisation and validation of clinically relevant disease biomarkers. Phage display offers many advantages compared to antibody generation by immunisation because it is an unlimited resource of affinity reagents without batch-to-batch variation and is also amendable for high throughput in contrast to conventional hybridoma technology. One of the major bottlenecks to proteome-wide binder selection is the limited supply of suitable target antigens representative of the human proteome. Here, we provide proof of principle of using easily accessible, cancer-associated protein epitope signature tags (PrESTs), routinely generated within the Human Protein Atlas project, as surrogate antigens for full-length proteins in phage selections for the retrieval of target-specific binders. These binders were subsequently tested in western blot, immunohistochemistry and protein microarray application to demonstrate their functionality.},
  author       = {Gustavsson, Elin and Ek, Sara and Steen, Johanna and Nordström, Malin and Älgenäs, Cajsa and Uhlén, Mathias and Wingren, Christer and Ottosson, Jenny and Hober, Sophia and Borrebaeck, Carl},
  issn         = {1876-4347},
  language     = {eng},
  pages        = {302--311},
  publisher    = {Elsevier},
  series       = {New Biotechnology},
  title        = {Surrogate antigens as targets for proteome-wide binder selection},
  url          = {http://dx.doi.org/10.1016/j.nbt.2010.12.005},
  volume       = {28},
  year         = {2011},
}