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Sialyl-Tn Antigen-Imprinted Dual Fluorescent Core-Shell Nanoparticles for Ratiometric Sialyl-Tn Antigen Detection and Dual-Color Labeling of Cancer Cells

Jiang, Shan ; Wang, Tianyan ; Behren, Sandra ; Westerlind, Ulrika ; Gawlitza, Kornelia ; Persson, Jenny L. LU and Rurack, Knut (2022) In ACS Applied Nano Materials 5(12). p.17592-17605
Abstract

Sialyl-Tn (STn or sialyl-Thomsen-nouveau) is a carbohydrate antigen expressed by more than 80% of human carcinomas. We here report a strategy for ratiometric STn detection and dual-color cancer cell labeling, particularly, by molecularly imprinted polymers (MIPs). Imprinting was based on spectroscopic studies of a urea-containing green-fluorescent monomer 1 and STn-Thr-Na (sodium salt of Neu5Acα2-6GalNAcα-O-Thr). A few-nanometer-thin green-fluorescent polymer shell, in which STn-Thr-Na was imprinted with 1, other comonomers, and a cross-linker, was synthesized from the surface of red-emissive carbon nanodot (R-CND)-doped silica nanoparticles, resulting in dual fluorescent STn-MIPs. Dual-color labeling of cancer cells was achieved since... (More)

Sialyl-Tn (STn or sialyl-Thomsen-nouveau) is a carbohydrate antigen expressed by more than 80% of human carcinomas. We here report a strategy for ratiometric STn detection and dual-color cancer cell labeling, particularly, by molecularly imprinted polymers (MIPs). Imprinting was based on spectroscopic studies of a urea-containing green-fluorescent monomer 1 and STn-Thr-Na (sodium salt of Neu5Acα2-6GalNAcα-O-Thr). A few-nanometer-thin green-fluorescent polymer shell, in which STn-Thr-Na was imprinted with 1, other comonomers, and a cross-linker, was synthesized from the surface of red-emissive carbon nanodot (R-CND)-doped silica nanoparticles, resulting in dual fluorescent STn-MIPs. Dual-color labeling of cancer cells was achieved since both red and green emissions were detected in two separate channels of the microscope and an improved accuracy was obtained in comparison with single-signal MIPs. The flow cytometric cell analysis showed that the binding of STn-MIPs was significantly higher (p < 0.001) than that of non-imprinted polymer (NIP) control particles within the same cell line, allowing to distinguish populations. Based on the modularity of the luminescent core-fluorescent MIP shell architecture, the concept can be transferred in a straightforward manner to other target analytes.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cancer, core-shell particles, dual-color labeling, glycan, molecular imprinting
in
ACS Applied Nano Materials
volume
5
issue
12
pages
14 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:36583127
  • scopus:85142609638
ISSN
2574-0970
DOI
10.1021/acsanm.2c03252
language
English
LU publication?
yes
id
17916880-c8bc-436c-b20a-f4b7cecbb78b
date added to LUP
2023-01-25 11:16:59
date last changed
2024-04-18 08:42:11
@article{17916880-c8bc-436c-b20a-f4b7cecbb78b,
  abstract     = {{<p>Sialyl-Tn (STn or sialyl-Thomsen-nouveau) is a carbohydrate antigen expressed by more than 80% of human carcinomas. We here report a strategy for ratiometric STn detection and dual-color cancer cell labeling, particularly, by molecularly imprinted polymers (MIPs). Imprinting was based on spectroscopic studies of a urea-containing green-fluorescent monomer 1 and STn-Thr-Na (sodium salt of Neu5Acα2-6GalNAcα-O-Thr). A few-nanometer-thin green-fluorescent polymer shell, in which STn-Thr-Na was imprinted with 1, other comonomers, and a cross-linker, was synthesized from the surface of red-emissive carbon nanodot (R-CND)-doped silica nanoparticles, resulting in dual fluorescent STn-MIPs. Dual-color labeling of cancer cells was achieved since both red and green emissions were detected in two separate channels of the microscope and an improved accuracy was obtained in comparison with single-signal MIPs. The flow cytometric cell analysis showed that the binding of STn-MIPs was significantly higher (p &lt; 0.001) than that of non-imprinted polymer (NIP) control particles within the same cell line, allowing to distinguish populations. Based on the modularity of the luminescent core-fluorescent MIP shell architecture, the concept can be transferred in a straightforward manner to other target analytes.</p>}},
  author       = {{Jiang, Shan and Wang, Tianyan and Behren, Sandra and Westerlind, Ulrika and Gawlitza, Kornelia and Persson, Jenny L. and Rurack, Knut}},
  issn         = {{2574-0970}},
  keywords     = {{cancer; core-shell particles; dual-color labeling; glycan; molecular imprinting}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{12}},
  pages        = {{17592--17605}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Applied Nano Materials}},
  title        = {{Sialyl-Tn Antigen-Imprinted Dual Fluorescent Core-Shell Nanoparticles for Ratiometric Sialyl-Tn Antigen Detection and Dual-Color Labeling of Cancer Cells}},
  url          = {{http://dx.doi.org/10.1021/acsanm.2c03252}},
  doi          = {{10.1021/acsanm.2c03252}},
  volume       = {{5}},
  year         = {{2022}},
}