An Amperometric Lactate Sensor Based on a NAD+-Analog and Lactate Dehydrogenase Coimmobilized on Reticulated Vitreous Carbon
(1997) In Electroanalysis 9(7). p.523-526- Abstract
- A NAD+-analog was coimmobilized with lactate dehydrogenase
(LDH) on reticulated vitreous carbon (RVC) to give an amperometric
lactate biosensor. Both LDH and the NAD+ -analog were bound
covalently with carbodiimide to the surface of the porous RVC-material.
The electrode was operated in a FIA-arrangement in the presence or
absence of a soluble mediator. Meldola Blue. The stability was poor when
the electrode was operated at +400 mV (vs. Ag/AgCl) in the absence of
mediator but improved most significantly in the presence of 5 μM
mediator, so that 65% of the original activity remained after 16 days.
The amperometric currents were smaller with regeneration by mediator at
... (More) - A NAD+-analog was coimmobilized with lactate dehydrogenase
(LDH) on reticulated vitreous carbon (RVC) to give an amperometric
lactate biosensor. Both LDH and the NAD+ -analog were bound
covalently with carbodiimide to the surface of the porous RVC-material.
The electrode was operated in a FIA-arrangement in the presence or
absence of a soluble mediator. Meldola Blue. The stability was poor when
the electrode was operated at +400 mV (vs. Ag/AgCl) in the absence of
mediator but improved most significantly in the presence of 5 μM
mediator, so that 65% of the original activity remained after 16 days.
The amperometric currents were smaller with regeneration by mediator at
−100 mV than with direct electrochemical oxidation at +400 mV,
indicating that the additional steps slow down the reaction rate. Linear
calibration plots were obtained from the detection limit, 1 μM, to 500
μM lactate with 5 μM mediator, reoxidized at −100 mV. The sample
throughput was about 60 h−1. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/17a7b3cc-8fb5-4c45-b576-3ac6ddd95bd6
- author
- Khayyami, Masoud ; Peña Garcia, Nuria ; Larsson, Per-Olof LU ; Danielsson, Bengt LU and Johansson, Gillis
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Electroanalysis
- volume
- 9
- issue
- 7
- pages
- 4 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:0000183811
- ISSN
- 1040-0397
- DOI
- 10.1002/elan.1140090703
- language
- English
- LU publication?
- yes
- id
- 17a7b3cc-8fb5-4c45-b576-3ac6ddd95bd6
- date added to LUP
- 2024-06-17 10:30:14
- date last changed
- 2024-06-18 04:01:26
@article{17a7b3cc-8fb5-4c45-b576-3ac6ddd95bd6, abstract = {{A NAD<sup>+</sup>-analog was coimmobilized with lactate dehydrogenase <br> (LDH) on reticulated vitreous carbon (RVC) to give an amperometric <br> lactate biosensor. Both LDH and the NAD<sup>+</sup> -analog were bound <br> covalently with carbodiimide to the surface of the porous RVC-material. <br> The electrode was operated in a FIA-arrangement in the presence or <br> absence of a soluble mediator. Meldola Blue. The stability was poor when<br> the electrode was operated at +400 mV (vs. Ag/AgCl) in the absence of <br> mediator but improved most significantly in the presence of 5 μM <br> mediator, so that 65% of the original activity remained after 16 days. <br> The amperometric currents were smaller with regeneration by mediator at <br> −100 mV than with direct electrochemical oxidation at +400 mV, <br> indicating that the additional steps slow down the reaction rate. Linear<br> calibration plots were obtained from the detection limit, 1 μM, to 500 <br> μM lactate with 5 μM mediator, reoxidized at −100 mV. The sample <br> throughput was about 60 h<sup>−1</sup>.}}, author = {{Khayyami, Masoud and Peña Garcia, Nuria and Larsson, Per-Olof and Danielsson, Bengt and Johansson, Gillis}}, issn = {{1040-0397}}, language = {{eng}}, number = {{7}}, pages = {{523--526}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Electroanalysis}}, title = {{An Amperometric Lactate Sensor Based on a NAD<sup>+</sup>-Analog and Lactate Dehydrogenase Coimmobilized on Reticulated Vitreous Carbon}}, url = {{http://dx.doi.org/10.1002/elan.1140090703}}, doi = {{10.1002/elan.1140090703}}, volume = {{9}}, year = {{1997}}, }