Complimentary action : C1q increases ganglion cell survival in an in vitro model of retinal degeneration
Taylor, Linnéa LU ; Arnér, Karin LU ; Blom, Anna M. LU
and Ghosh, Fredrik
LU
(2016)
In Journal of Neuroimmunology
298.
p.117-129
- Abstract
Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50 nM), C1-inhibitor (C1-inh; Berinert; 500 mg/l), C1q + C1-inh, and no supplementation (culture controls). Explants were kept for 12 h or 2 days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production... (More)
Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50 nM), C1-inhibitor (C1-inh; Berinert; 500 mg/l), C1q + C1-inh, and no supplementation (culture controls). Explants were kept for 12 h or 2 days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production of C1q, and a quiesced gliotic response.
(Less)
- author
- Taylor, Linnéa
LU
; Arnér, Karin
LU
; Blom, Anna M.
LU
and Ghosh, Fredrik
LU
- organization
- publishing date
- 2016-09-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adult retina, C1q, Ganglion cell, Neuroinflammation, Retinal degeneration, Retinal explant culture
- in
- Journal of Neuroimmunology
- volume
- 298
- pages
- 13 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:27609284
- wos:000383936600017
- scopus:84978957733
- ISSN
- 0165-5728
- DOI
- 10.1016/j.jneuroim.2016.07.014
- language
- English
- LU publication?
- yes
- id
- 17c002b3-e318-4861-9033-2045be6926c6
- date added to LUP
- 2016-08-18 16:38:04
- date last changed
- 2025-03-08 14:27:17
@article{17c002b3-e318-4861-9033-2045be6926c6,
abstract = {{<p>Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50 nM), C1-inhibitor (C1-inh; Berinert; 500 mg/l), C1q + C1-inh, and no supplementation (culture controls). Explants were kept for 12 h or 2 days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production of C1q, and a quiesced gliotic response.</p>}},
author = {{Taylor, Linnéa and Arnér, Karin and Blom, Anna M. and Ghosh, Fredrik}},
issn = {{0165-5728}},
keywords = {{Adult retina; C1q; Ganglion cell; Neuroinflammation; Retinal degeneration; Retinal explant culture}},
language = {{eng}},
month = {{09}},
pages = {{117--129}},
publisher = {{Elsevier}},
series = {{Journal of Neuroimmunology}},
title = {{Complimentary action : C1q increases ganglion cell survival in an in vitro model of retinal degeneration}},
url = {{http://dx.doi.org/10.1016/j.jneuroim.2016.07.014}},
doi = {{10.1016/j.jneuroim.2016.07.014}},
volume = {{298}},
year = {{2016}},
}