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Complimentary action : C1q increases ganglion cell survival in an in vitro model of retinal degeneration

Taylor, Linnéa LU ; Arnér, Karin LU ; Blom, Anna M. LU and Ghosh, Fredrik LU (2016) In Journal of Neuroimmunology 298. p.117-129
Abstract

Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50 nM), C1-inhibitor (C1-inh; Berinert; 500 mg/l), C1q + C1-inh, and no supplementation (culture controls). Explants were kept for 12 h or 2 days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production... (More)

Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50 nM), C1-inhibitor (C1-inh; Berinert; 500 mg/l), C1q + C1-inh, and no supplementation (culture controls). Explants were kept for 12 h or 2 days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production of C1q, and a quiesced gliotic response.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adult retina, C1q, Ganglion cell, Neuroinflammation, Retinal degeneration, Retinal explant culture
in
Journal of Neuroimmunology
volume
298
pages
13 pages
publisher
Elsevier
external identifiers
  • Scopus:84978957733
  • WOS:000383936600017
ISSN
0165-5728
DOI
10.1016/j.jneuroim.2016.07.014
language
English
LU publication?
yes
id
17c002b3-e318-4861-9033-2045be6926c6
date added to LUP
2016-08-18 16:38:04
date last changed
2017-01-01 08:32:06
@article{17c002b3-e318-4861-9033-2045be6926c6,
  abstract     = {<p>Using a previously described retinal explant culture system as an acute injury model, we here explore the role of C1q, the initiator of the classical complement pathway, in neuronal cell survival and retinal homeostasis. Full-thickness adult rat retinal explants were divided into four groups, receiving the following supplementation: C1q (50 nM), C1-inhibitor (C1-inh; Berinert; 500 mg/l), C1q + C1-inh, and no supplementation (culture controls). Explants were kept for 12 h or 2 days after which they were examined morphologically and with a panel of immunohistochemical markers. C1q supplementation protects ganglion cells from degeneration within the explant in vitro system. This effect is correlated to an attenuated endogenous production of C1q, and a quiesced gliotic response.</p>},
  author       = {Taylor, Linnéa and Arnér, Karin and Blom, Anna M. and Ghosh, Fredrik},
  issn         = {0165-5728},
  keyword      = {Adult retina,C1q,Ganglion cell,Neuroinflammation,Retinal degeneration,Retinal explant culture},
  language     = {eng},
  month        = {09},
  pages        = {117--129},
  publisher    = {Elsevier},
  series       = {Journal of Neuroimmunology},
  title        = {Complimentary action : C1q increases ganglion cell survival in an in vitro model of retinal degeneration},
  url          = {http://dx.doi.org/10.1016/j.jneuroim.2016.07.014},
  volume       = {298},
  year         = {2016},
}