Advanced

Effect of bilayer charge on lipoprotein lipid exchange

Browning, Kathryn Louise ; Lind, Tania Kjellerup LU ; Maric, Selma LU ; Barker, Robert David ; Cárdenas, Marité LU and Malmsten, Martin LU (2018) In Colloids and Surfaces. B, Biointerfaces 168. p.117-125
Abstract

Lipoproteins play a key role in the onset and development of atherosclerosis, the formation of lipid plaques at blood vessel walls. The plaque formation, as well as subsequent calcification, involves not only endothelial cells but also connective tissue, and is closely related to a wide range of cardiovascular syndromes, that together constitute the number one cause of death in the Western World. High (HDL) and low (LDL) density lipoproteins are of particular interest in relation to atherosclerosis, due to their protective and harmful effects, respectively. In an effort to elucidate the molecular mechanisms underlying this, and to identify factors determining lipid deposition and exchange at lipid membranes, we here employ neutron... (More)

Lipoproteins play a key role in the onset and development of atherosclerosis, the formation of lipid plaques at blood vessel walls. The plaque formation, as well as subsequent calcification, involves not only endothelial cells but also connective tissue, and is closely related to a wide range of cardiovascular syndromes, that together constitute the number one cause of death in the Western World. High (HDL) and low (LDL) density lipoproteins are of particular interest in relation to atherosclerosis, due to their protective and harmful effects, respectively. In an effort to elucidate the molecular mechanisms underlying this, and to identify factors determining lipid deposition and exchange at lipid membranes, we here employ neutron reflection (NR) and quartz crystal microbalance with dissipation (QCM-D) to study the effect of membrane charge on lipoprotein deposition and lipid exchange. Dimyristoylphosphatidylcholine (DMPC) bilayers containing varying amounts of negatively charged dimyristoylphosphatidylserine (DMPS) were used to vary membrane charge. It was found that the amount of hydrogenous material deposited from either HDL or LDL to the bilayer depends only weakly on membrane charge density. In contrast, increasing membrane charge resulted in an increase in the amount of lipids removed from the supported lipid bilayer, an effect particularly pronounced for LDL. The latter effects are in line with previously reported observations on atherosclerotic plaque prone regions of long-term hyperlipidaemia and type 2 diabetic patients, and may also provide some molecular clues into the relation between oxidative stress and atherosclerosis.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
in
Colloids and Surfaces. B, Biointerfaces
volume
168
pages
9 pages
publisher
Elsevier
external identifiers
  • pmid:29422308
  • scopus:85041618999
ISSN
1873-4367
DOI
10.1016/j.colsurfb.2018.01.043
language
English
LU publication?
no
id
17c10f5c-04fb-4642-ada0-b78060943abd
date added to LUP
2018-07-19 12:01:14
date last changed
2021-04-06 01:49:57
@article{17c10f5c-04fb-4642-ada0-b78060943abd,
  abstract     = {<p>Lipoproteins play a key role in the onset and development of atherosclerosis, the formation of lipid plaques at blood vessel walls. The plaque formation, as well as subsequent calcification, involves not only endothelial cells but also connective tissue, and is closely related to a wide range of cardiovascular syndromes, that together constitute the number one cause of death in the Western World. High (HDL) and low (LDL) density lipoproteins are of particular interest in relation to atherosclerosis, due to their protective and harmful effects, respectively. In an effort to elucidate the molecular mechanisms underlying this, and to identify factors determining lipid deposition and exchange at lipid membranes, we here employ neutron reflection (NR) and quartz crystal microbalance with dissipation (QCM-D) to study the effect of membrane charge on lipoprotein deposition and lipid exchange. Dimyristoylphosphatidylcholine (DMPC) bilayers containing varying amounts of negatively charged dimyristoylphosphatidylserine (DMPS) were used to vary membrane charge. It was found that the amount of hydrogenous material deposited from either HDL or LDL to the bilayer depends only weakly on membrane charge density. In contrast, increasing membrane charge resulted in an increase in the amount of lipids removed from the supported lipid bilayer, an effect particularly pronounced for LDL. The latter effects are in line with previously reported observations on atherosclerotic plaque prone regions of long-term hyperlipidaemia and type 2 diabetic patients, and may also provide some molecular clues into the relation between oxidative stress and atherosclerosis.</p>},
  author       = {Browning, Kathryn Louise and Lind, Tania Kjellerup and Maric, Selma and Barker, Robert David and Cárdenas, Marité and Malmsten, Martin},
  issn         = {1873-4367},
  language     = {eng},
  month        = {08},
  pages        = {117--125},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces. B, Biointerfaces},
  title        = {Effect of bilayer charge on lipoprotein lipid exchange},
  url          = {http://dx.doi.org/10.1016/j.colsurfb.2018.01.043},
  doi          = {10.1016/j.colsurfb.2018.01.043},
  volume       = {168},
  year         = {2018},
}