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A beta 40 Oligomers Identified as a Potential Biomarker for the Diagnosis of Alzheimer's Disease

Gao, Carol Man; Yam, Alice Y.; Wang, Xuemei; Magdangal, Erika; Salisbury, Cleo; Peretz, David; Zuckermann, Ronald N.; Connolly, Michael D.; Hansson, Oskar LU and Minthon, Lennart LU , et al. (2010) In PLoS ONE 5(12).
Abstract
Alzheimer's Disease (AD) is the most prevalent form of dementia worldwide, yet the development of therapeutics has been hampered by the absence of suitable biomarkers to diagnose the disease in its early stages prior to the formation of amyloid plaques and the occurrence of irreversible neuronal damage. Since oligomeric A beta species have been implicated in the pathophysiology of AD, we reasoned that they may correlate with the onset of disease. As such, we have developed a novel misfolded protein assay for the detection of soluble oligomers composed of A beta x-40 and x-42 peptide (hereafter A beta 40 and A beta 42) from cerebrospinal fluid (CSF). Preliminary validation of this assay with 36 clinical samples demonstrated the presence of... (More)
Alzheimer's Disease (AD) is the most prevalent form of dementia worldwide, yet the development of therapeutics has been hampered by the absence of suitable biomarkers to diagnose the disease in its early stages prior to the formation of amyloid plaques and the occurrence of irreversible neuronal damage. Since oligomeric A beta species have been implicated in the pathophysiology of AD, we reasoned that they may correlate with the onset of disease. As such, we have developed a novel misfolded protein assay for the detection of soluble oligomers composed of A beta x-40 and x-42 peptide (hereafter A beta 40 and A beta 42) from cerebrospinal fluid (CSF). Preliminary validation of this assay with 36 clinical samples demonstrated the presence of aggregated A beta 40 in the CSF of AD patients. Together with measurements of total A beta 42, diagnostic sensitivity and specificity greater than 95% and 90%, respectively, were achieved. Although larger sample populations will be needed to confirm this diagnostic sensitivity, our studies demonstrate a sensitive method of detecting circulating A beta 40 oligomers from AD CSF and suggest that these oligomers could be a powerful new biomarker for the early detection of AD. (Less)
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PLoS ONE
volume
5
issue
12
publisher
Public Library of Science
external identifiers
  • wos:000285793600030
  • scopus:78651269012
ISSN
1932-6203
DOI
10.1371/journal.pone.0015725
language
English
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yes
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727d4677-2b34-46b7-9473-6902ae8b5b3a (old id 1810927)
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2011-03-02 14:15:37
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@article{727d4677-2b34-46b7-9473-6902ae8b5b3a,
  abstract     = {Alzheimer's Disease (AD) is the most prevalent form of dementia worldwide, yet the development of therapeutics has been hampered by the absence of suitable biomarkers to diagnose the disease in its early stages prior to the formation of amyloid plaques and the occurrence of irreversible neuronal damage. Since oligomeric A beta species have been implicated in the pathophysiology of AD, we reasoned that they may correlate with the onset of disease. As such, we have developed a novel misfolded protein assay for the detection of soluble oligomers composed of A beta x-40 and x-42 peptide (hereafter A beta 40 and A beta 42) from cerebrospinal fluid (CSF). Preliminary validation of this assay with 36 clinical samples demonstrated the presence of aggregated A beta 40 in the CSF of AD patients. Together with measurements of total A beta 42, diagnostic sensitivity and specificity greater than 95% and 90%, respectively, were achieved. Although larger sample populations will be needed to confirm this diagnostic sensitivity, our studies demonstrate a sensitive method of detecting circulating A beta 40 oligomers from AD CSF and suggest that these oligomers could be a powerful new biomarker for the early detection of AD.},
  author       = {Gao, Carol Man and Yam, Alice Y. and Wang, Xuemei and Magdangal, Erika and Salisbury, Cleo and Peretz, David and Zuckermann, Ronald N. and Connolly, Michael D. and Hansson, Oskar and Minthon, Lennart and Zetterberg, Henrik and Blennow, Kaj and Fedynyshyn, Joseph P. and Allauzen, Sophie},
  issn         = {1932-6203},
  language     = {eng},
  number       = {12},
  publisher    = {Public Library of Science},
  series       = {PLoS ONE},
  title        = {A beta 40 Oligomers Identified as a Potential Biomarker for the Diagnosis of Alzheimer's Disease},
  url          = {http://dx.doi.org/10.1371/journal.pone.0015725},
  volume       = {5},
  year         = {2010},
}