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Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice

Marco, Maria L.; de Vries, Maaike C.; Wels, Michiel; Molenaar, Douwe; Mangell, Peter LU ; Ahrné, Siv LU ; de Vos, Willem M.; Vaughan, Elaine E. and Kleerebezem, Michiel (2010) In The Isme Journal 4(11). p.1481-1484
Abstract
Probiotic bacteria provide unique opportunities to study the global responses and molecular mechanisms underlying the effects of gut-associated microorganisms in the human digestive tract. In this study, we show by comparative transcriptome analysis using DNA microarrays that the established probiotic Lactobacillus plantarum 299v specifically adapts its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and protein-aceous cell surface compounds. This report constitutes the first application of global gene expression profiling of a commensal microorganism in the human gut. A core L. plantarum transcriptome expressed in the mammalian intestine was also determined through comparisons of... (More)
Probiotic bacteria provide unique opportunities to study the global responses and molecular mechanisms underlying the effects of gut-associated microorganisms in the human digestive tract. In this study, we show by comparative transcriptome analysis using DNA microarrays that the established probiotic Lactobacillus plantarum 299v specifically adapts its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and protein-aceous cell surface compounds. This report constitutes the first application of global gene expression profiling of a commensal microorganism in the human gut. A core L. plantarum transcriptome expressed in the mammalian intestine was also determined through comparisons of L. plantarum 299v activities in humans to those found for L. plantarum WCFS1 in germ-free mice. These results identify the niche-specific adaptations of a dietary microorganism to the intestinal ecosystem and provide novel targets for molecular analysis of microbial-host interactions which affect human health. The ISME Journal (2010) 4, 1481-1484; doi: 10.1038/ismej.2010.61; published online 27 May 2010 (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
intestine, transcript profiling, germ-free mice, gut microbiota, diet, in vivo-inducible genes
in
The Isme Journal
volume
4
issue
11
pages
1481 - 1484
publisher
Nature Publishing Group
external identifiers
  • wos:000285793700012
  • scopus:77958455790
ISSN
1751-7362
DOI
10.1038/ismej.2010.61
language
English
LU publication?
yes
id
7b75b14e-e907-4552-988a-408463101b9c (old id 1814884)
date added to LUP
2011-03-02 12:59:50
date last changed
2018-06-24 03:07:12
@article{7b75b14e-e907-4552-988a-408463101b9c,
  abstract     = {Probiotic bacteria provide unique opportunities to study the global responses and molecular mechanisms underlying the effects of gut-associated microorganisms in the human digestive tract. In this study, we show by comparative transcriptome analysis using DNA microarrays that the established probiotic Lactobacillus plantarum 299v specifically adapts its metabolic capacity in the human intestine for carbohydrate acquisition and expression of exopolysaccharide and protein-aceous cell surface compounds. This report constitutes the first application of global gene expression profiling of a commensal microorganism in the human gut. A core L. plantarum transcriptome expressed in the mammalian intestine was also determined through comparisons of L. plantarum 299v activities in humans to those found for L. plantarum WCFS1 in germ-free mice. These results identify the niche-specific adaptations of a dietary microorganism to the intestinal ecosystem and provide novel targets for molecular analysis of microbial-host interactions which affect human health. The ISME Journal (2010) 4, 1481-1484; doi: 10.1038/ismej.2010.61; published online 27 May 2010},
  author       = {Marco, Maria L. and de Vries, Maaike C. and Wels, Michiel and Molenaar, Douwe and Mangell, Peter and Ahrné, Siv and de Vos, Willem M. and Vaughan, Elaine E. and Kleerebezem, Michiel},
  issn         = {1751-7362},
  keyword      = {intestine,transcript profiling,germ-free mice,gut microbiota,diet,in vivo-inducible genes},
  language     = {eng},
  number       = {11},
  pages        = {1481--1484},
  publisher    = {Nature Publishing Group},
  series       = {The Isme Journal},
  title        = {Convergence in probiotic Lactobacillus gut-adaptive responses in humans and mice},
  url          = {http://dx.doi.org/10.1038/ismej.2010.61},
  volume       = {4},
  year         = {2010},
}