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Alternative lengthening of telomeres-An enhanced chromosomal instability in aggressive non-MYCN amplified and telomere elongated neuroblastomas.

Lundberg, Gisela LU ; Sehic, Daniel LU ; Länsberg, John-Kalle LU ; Øra, Ingrid LU ; Frigyesi, Attila LU ; Castel, Victoria; Navarro, Samuel; Piqueras, Marta; Martinsson, Tommy and Noguera, Rosa, et al. (2011) In Genes, Chromosomes and Cancer 50(4). p.250-262
Abstract
Telomere length alterations are known to cause genomic instability and influence clinical course in several tumor types, but have been little investigated in neuroblastoma (NB), one of the most common childhood tumors. In the present study, telomere-dependent chromosomal instability and telomere length were determined in six NB cell lines and fifty tumor biopsies. The alternative lengthening of telomeres (ALT) pathway was assayed by scoring ALT-associated promyelocytic leukemia (PML) bodies (APBs). We found a reduced probability of overall survival for tumors with increased telomere length compared to cases with reduced or unchanged telomere length. In non-MYCN amplified tumors, a reduced or unchanged telomere length was associated with... (More)
Telomere length alterations are known to cause genomic instability and influence clinical course in several tumor types, but have been little investigated in neuroblastoma (NB), one of the most common childhood tumors. In the present study, telomere-dependent chromosomal instability and telomere length were determined in six NB cell lines and fifty tumor biopsies. The alternative lengthening of telomeres (ALT) pathway was assayed by scoring ALT-associated promyelocytic leukemia (PML) bodies (APBs). We found a reduced probability of overall survival for tumors with increased telomere length compared to cases with reduced or unchanged telomere length. In non-MYCN amplified tumors, a reduced or unchanged telomere length was associated with 100% overall survival. Tumor cells with increased telomere length had an elevated frequency of APBs, consistent with activation of the ALT pathway. The vast majority of tumor biopsies and cell lines exhibited an elevated rate of anaphase bridges, suggesting telomere-dependent chromosomal instability. This was more pronounced in tumors with increased telomere length. In cell lines, there was a close correlation between lack of telomere-protective TTAGGG-repeats, anaphase bridging, and remodeling of oncogene sequences. Thus, telomere-dependent chromosomal instability is highly prevalent in NB, and may contribute to the complexity of genomic alterations as well as therapy resistance in the absence of MYCN amplification and in this tumor type. © 2011 Wiley-Liss, Inc. (Less)
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Genes, Chromosomes and Cancer
volume
50
issue
4
pages
250 - 262
publisher
John Wiley & Sons
external identifiers
  • wos:000287261000005
  • pmid:21319260
  • scopus:79851490466
ISSN
1045-2257
DOI
10.1002/gcc.20850
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English
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yes
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2f7ac315-b0e8-4a41-96cb-d1001d7d845a (old id 1831846)
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http://www.ncbi.nlm.nih.gov/pubmed/21319260?dopt=Abstract
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2011-03-01 14:52:24
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@article{2f7ac315-b0e8-4a41-96cb-d1001d7d845a,
  abstract     = {Telomere length alterations are known to cause genomic instability and influence clinical course in several tumor types, but have been little investigated in neuroblastoma (NB), one of the most common childhood tumors. In the present study, telomere-dependent chromosomal instability and telomere length were determined in six NB cell lines and fifty tumor biopsies. The alternative lengthening of telomeres (ALT) pathway was assayed by scoring ALT-associated promyelocytic leukemia (PML) bodies (APBs). We found a reduced probability of overall survival for tumors with increased telomere length compared to cases with reduced or unchanged telomere length. In non-MYCN amplified tumors, a reduced or unchanged telomere length was associated with 100% overall survival. Tumor cells with increased telomere length had an elevated frequency of APBs, consistent with activation of the ALT pathway. The vast majority of tumor biopsies and cell lines exhibited an elevated rate of anaphase bridges, suggesting telomere-dependent chromosomal instability. This was more pronounced in tumors with increased telomere length. In cell lines, there was a close correlation between lack of telomere-protective TTAGGG-repeats, anaphase bridging, and remodeling of oncogene sequences. Thus, telomere-dependent chromosomal instability is highly prevalent in NB, and may contribute to the complexity of genomic alterations as well as therapy resistance in the absence of MYCN amplification and in this tumor type. © 2011 Wiley-Liss, Inc.},
  author       = {Lundberg, Gisela and Sehic, Daniel and Länsberg, John-Kalle and Øra, Ingrid and Frigyesi, Attila and Castel, Victoria and Navarro, Samuel and Piqueras, Marta and Martinsson, Tommy and Noguera, Rosa and Gisselsson Nord, David},
  issn         = {1045-2257},
  language     = {eng},
  number       = {4},
  pages        = {250--262},
  publisher    = {John Wiley & Sons},
  series       = {Genes, Chromosomes and Cancer},
  title        = {Alternative lengthening of telomeres-An enhanced chromosomal instability in aggressive non-MYCN amplified and telomere elongated neuroblastomas.},
  url          = {http://dx.doi.org/10.1002/gcc.20850},
  volume       = {50},
  year         = {2011},
}