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Two novel classes of enzymes are required for the biosynthesis of aurofusarin in Fusarium graminearum.

Frandsen, Rasmus J N; Schutt, Claes; Lund, Birgitte W; Staerk, Dan; Nielsen, John; Olsson Hau, Stefan LU and Giese, Henriette (2011) In Journal of Biological Chemistry
Abstract
Previous studies have reported the functional characterization of nine out of eleven genes found in the gene cluster responsible for biosynthesis of the polyketide pigment aurofusarin in Fusarium graminearum. Here we reanalyse the function of a putative aurofusarin pump (AurT) and the two remaining orphan genes, aurZ and aurS. Targeted gene replacement of aurZ resulted in the discovery that the compound YWA1, rather than nor-rubrofusarin, is the primary product of F. graminearum polyketide synthase 12 (FgPKS12). AurZ is the first representative of a novel class of dehydratases that act on hydroxylated γ-pyrones. Replacement of the aurS gene resulted in accumulation of rubrofusarin, an intermediate that also accumulates when the GIP1, aurF... (More)
Previous studies have reported the functional characterization of nine out of eleven genes found in the gene cluster responsible for biosynthesis of the polyketide pigment aurofusarin in Fusarium graminearum. Here we reanalyse the function of a putative aurofusarin pump (AurT) and the two remaining orphan genes, aurZ and aurS. Targeted gene replacement of aurZ resulted in the discovery that the compound YWA1, rather than nor-rubrofusarin, is the primary product of F. graminearum polyketide synthase 12 (FgPKS12). AurZ is the first representative of a novel class of dehydratases that act on hydroxylated γ-pyrones. Replacement of the aurS gene resulted in accumulation of rubrofusarin, an intermediate that also accumulates when the GIP1, aurF or aurO genes in the aurofusarin cluster are deleted. Based on the shared phenotype and predicted subcellular localization we propose that AurS is a member of an extracellular enzyme complex (GIP1-AurF-AurO-AurS) responsible for converting rubrofusarin into aurofusarin. This implies that rubrofusarin, rather than aurofusarin, is pumped across the plasma membrane. Replacement of the putative aurofusarin pump aurT increased rubrofusarin to aurofusarin ratio, supporting that rubrofusarin is normally pumped across the plasma membrane. These results provide functional information on two novel classes of proteins and their contribution to polyketide pigment biosynthesis. (Less)
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Contribution to journal
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published
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in
Journal of Biological Chemistry
publisher
ASBMB
external identifiers
  • pmid:21296881
  • scopus:79953188685
ISSN
1083-351X
DOI
10.1074/jbc.M110.179853
language
English
LU publication?
yes
id
6b1372fe-808f-46a3-932c-f2c754a562a2 (old id 1832248)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21296881?dopt=Abstract
date added to LUP
2011-03-01 12:50:56
date last changed
2017-04-16 04:22:24
@article{6b1372fe-808f-46a3-932c-f2c754a562a2,
  abstract     = {Previous studies have reported the functional characterization of nine out of eleven genes found in the gene cluster responsible for biosynthesis of the polyketide pigment aurofusarin in Fusarium graminearum. Here we reanalyse the function of a putative aurofusarin pump (AurT) and the two remaining orphan genes, aurZ and aurS. Targeted gene replacement of aurZ resulted in the discovery that the compound YWA1, rather than nor-rubrofusarin, is the primary product of F. graminearum polyketide synthase 12 (FgPKS12). AurZ is the first representative of a novel class of dehydratases that act on hydroxylated γ-pyrones. Replacement of the aurS gene resulted in accumulation of rubrofusarin, an intermediate that also accumulates when the GIP1, aurF or aurO genes in the aurofusarin cluster are deleted. Based on the shared phenotype and predicted subcellular localization we propose that AurS is a member of an extracellular enzyme complex (GIP1-AurF-AurO-AurS) responsible for converting rubrofusarin into aurofusarin. This implies that rubrofusarin, rather than aurofusarin, is pumped across the plasma membrane. Replacement of the putative aurofusarin pump aurT increased rubrofusarin to aurofusarin ratio, supporting that rubrofusarin is normally pumped across the plasma membrane. These results provide functional information on two novel classes of proteins and their contribution to polyketide pigment biosynthesis.},
  author       = {Frandsen, Rasmus J N and Schutt, Claes and Lund, Birgitte W and Staerk, Dan and Nielsen, John and Olsson Hau, Stefan and Giese, Henriette},
  issn         = {1083-351X},
  language     = {eng},
  month        = {02},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Two novel classes of enzymes are required for the biosynthesis of aurofusarin in Fusarium graminearum.},
  url          = {http://dx.doi.org/10.1074/jbc.M110.179853},
  year         = {2011},
}