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Endoplasmic reticulum dynamics in hippocampal dendritic spines induced by agonists of type I metabotropic glutamate but not by muscarinic acetylcholine receptors.

Ng, Ai Na LU and Toresson, Håkan LU (2011) In Synapse 65(4). p.351-355
Abstract
Neurons in the hippocampus exhibit subpopulations of dendritic spines that contain endoplasmic reticulum (ER). ER in spines is important for synaptic activity and its associated Ca(2+) signaling. The dynamic distribution of ER to spines is regulated by diacylglycerol and partly mediated by protein kinase C, metalloproteinases and γ-secretase. In this study, we explored whether pharmacological activation of type I metabotropic glutamate receptors (mGluRs) and muscarinic acetylcholine receptors (mAChRs) known to activate phospholipase C would have any effect on spine ER content. We found that DHPG (100 μM) but not carbachol (10 μM) caused a reduction in the number of spines with ER. We further found that ER Ca(2+) depletion triggered by... (More)
Neurons in the hippocampus exhibit subpopulations of dendritic spines that contain endoplasmic reticulum (ER). ER in spines is important for synaptic activity and its associated Ca(2+) signaling. The dynamic distribution of ER to spines is regulated by diacylglycerol and partly mediated by protein kinase C, metalloproteinases and γ-secretase. In this study, we explored whether pharmacological activation of type I metabotropic glutamate receptors (mGluRs) and muscarinic acetylcholine receptors (mAChRs) known to activate phospholipase C would have any effect on spine ER content. We found that DHPG (100 μM) but not carbachol (10 μM) caused a reduction in the number of spines with ER. We further found that ER Ca(2+) depletion triggered by thapsigargin (200 nM) had no effect on ER localization in spines. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Synapse
volume
65
issue
4
pages
351 - 355
publisher
Wiley-Liss Inc.
external identifiers
  • wos:000287614000010
  • pmid:21284010
  • scopus:79251569260
ISSN
1098-2396
DOI
10.1002/syn.20887
language
English
LU publication?
yes
id
ef543bf6-9c8b-4f54-94fe-1e77a1cef9bc (old id 1832407)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21284010?dopt=Abstract
date added to LUP
2011-03-01 11:11:22
date last changed
2017-01-08 05:27:39
@article{ef543bf6-9c8b-4f54-94fe-1e77a1cef9bc,
  abstract     = {Neurons in the hippocampus exhibit subpopulations of dendritic spines that contain endoplasmic reticulum (ER). ER in spines is important for synaptic activity and its associated Ca(2+) signaling. The dynamic distribution of ER to spines is regulated by diacylglycerol and partly mediated by protein kinase C, metalloproteinases and γ-secretase. In this study, we explored whether pharmacological activation of type I metabotropic glutamate receptors (mGluRs) and muscarinic acetylcholine receptors (mAChRs) known to activate phospholipase C would have any effect on spine ER content. We found that DHPG (100 μM) but not carbachol (10 μM) caused a reduction in the number of spines with ER. We further found that ER Ca(2+) depletion triggered by thapsigargin (200 nM) had no effect on ER localization in spines.},
  author       = {Ng, Ai Na and Toresson, Håkan},
  issn         = {1098-2396},
  language     = {eng},
  number       = {4},
  pages        = {351--355},
  publisher    = {Wiley-Liss Inc.},
  series       = {Synapse},
  title        = {Endoplasmic reticulum dynamics in hippocampal dendritic spines induced by agonists of type I metabotropic glutamate but not by muscarinic acetylcholine receptors.},
  url          = {http://dx.doi.org/10.1002/syn.20887},
  volume       = {65},
  year         = {2011},
}