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Toward Next Generation Plasma Profiling via Heat-induced Epitope Retrieval and Array-based Assays

Schwenk, Jochen M.; Igel, Ulrika; Neiman, Maja; Langen, Hanno; Becker, Charlotte LU ; Bjartell, Anders LU ; Ponten, Fredrik; Wiklund, Fredrik; Gronberg, Henrik and Nilsson, Peter, et al. (2010) In Molecular & Cellular Proteomics 9(11). p.2497-2507
Abstract
There is a need for high throughput methods for screening patient samples in the quest for potential biomarkers for diagnostics and patient care. Here, we used a combination of undirected target selection, antibody suspension bead arrays, and heat-induced epitope retrieval to allow for protein profiling of human plasma in a novel and systematic manner. Several antibodies were found to reveal altered protein profiles upon epitope retrieval at elevated temperatures with limits of detection improving into lower ng/ml ranges. In a study based on prostate cancer patients, several proteins with differential profiles were discovered and subsequently validated in an independent cohort. For one of the potential biomarkers, the human carnosine... (More)
There is a need for high throughput methods for screening patient samples in the quest for potential biomarkers for diagnostics and patient care. Here, we used a combination of undirected target selection, antibody suspension bead arrays, and heat-induced epitope retrieval to allow for protein profiling of human plasma in a novel and systematic manner. Several antibodies were found to reveal altered protein profiles upon epitope retrieval at elevated temperatures with limits of detection improving into lower ng/ml ranges. In a study based on prostate cancer patients, several proteins with differential profiles were discovered and subsequently validated in an independent cohort. For one of the potential biomarkers, the human carnosine dipeptidase 1 protein (CNDP1), the differences were determined to be related to the glycosylation status of the targeted protein. The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format. Molecular & Cellular Proteomics 9:2497-2507, 2010. (Less)
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published
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in
Molecular & Cellular Proteomics
volume
9
issue
11
pages
2497 - 2507
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000285055900013
  • scopus:78149323414
ISSN
1535-9484
DOI
10.1074/mcp.M110.001560
language
English
LU publication?
yes
id
886eae47-ada6-4391-9087-a0464f897b7c (old id 1868051)
date added to LUP
2011-04-06 14:18:27
date last changed
2018-05-29 10:12:26
@article{886eae47-ada6-4391-9087-a0464f897b7c,
  abstract     = {There is a need for high throughput methods for screening patient samples in the quest for potential biomarkers for diagnostics and patient care. Here, we used a combination of undirected target selection, antibody suspension bead arrays, and heat-induced epitope retrieval to allow for protein profiling of human plasma in a novel and systematic manner. Several antibodies were found to reveal altered protein profiles upon epitope retrieval at elevated temperatures with limits of detection improving into lower ng/ml ranges. In a study based on prostate cancer patients, several proteins with differential profiles were discovered and subsequently validated in an independent cohort. For one of the potential biomarkers, the human carnosine dipeptidase 1 protein (CNDP1), the differences were determined to be related to the glycosylation status of the targeted protein. The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format. Molecular & Cellular Proteomics 9:2497-2507, 2010.},
  author       = {Schwenk, Jochen M. and Igel, Ulrika and Neiman, Maja and Langen, Hanno and Becker, Charlotte and Bjartell, Anders and Ponten, Fredrik and Wiklund, Fredrik and Gronberg, Henrik and Nilsson, Peter and Uhlen, Mathias},
  issn         = {1535-9484},
  language     = {eng},
  number       = {11},
  pages        = {2497--2507},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular & Cellular Proteomics},
  title        = {Toward Next Generation Plasma Profiling via Heat-induced Epitope Retrieval and Array-based Assays},
  url          = {http://dx.doi.org/10.1074/mcp.M110.001560},
  volume       = {9},
  year         = {2010},
}