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Coomassie Staining as Loading Control in Western Blot Analysis

Welinder, Charlotte LU and Ekblad, Lars LU (2011) In Journal of Proteome Research 10(3). p.1416-1419
Abstract
In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Western blot, loading control, Coomassie, MALDI-TOF, PVDF, GAPDH, peptide mass fingerprinting
in
Journal of Proteome Research
volume
10
issue
3
pages
1416 - 1419
publisher
The American Chemical Society
external identifiers
  • wos:000287944000044
  • scopus:79952400632
ISSN
1535-3893
DOI
10.1021/pr1011476
language
English
LU publication?
yes
id
bc5d1c6b-57f5-48f7-b597-b6f48d922d87 (old id 1869139)
date added to LUP
2011-04-04 09:39:36
date last changed
2017-10-29 03:22:58
@article{bc5d1c6b-57f5-48f7-b597-b6f48d922d87,
  abstract     = {In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.},
  author       = {Welinder, Charlotte and Ekblad, Lars},
  issn         = {1535-3893},
  keyword      = {Western blot,loading control,Coomassie,MALDI-TOF,PVDF,GAPDH,peptide mass fingerprinting},
  language     = {eng},
  number       = {3},
  pages        = {1416--1419},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Coomassie Staining as Loading Control in Western Blot Analysis},
  url          = {http://dx.doi.org/10.1021/pr1011476},
  volume       = {10},
  year         = {2011},
}