Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates
(2011) In Journal of Microbiological Methods 84(2). p.183-188- Abstract
- Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis... (More)
- Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1869174
- author
- Ryberg, Anna ; Olsson, Crister LU ; Ahrné, Siv LU and Monstein, Hans-Juerg
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Klebsiella spp., Molecular typing, (GTG)(5)-PCR, Ribosomal intergenic, transcribed spacer (ITS)-PCR, Fingerprint analysis, Multiple, displacement amplification
- in
- Journal of Microbiological Methods
- volume
- 84
- issue
- 2
- pages
- 183 - 188
- publisher
- Elsevier
- external identifiers
-
- wos:000287951200005
- scopus:79251613122
- pmid:21129413
- ISSN
- 1872-8359
- DOI
- 10.1016/j.mimet.2010.11.019
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Applied Nutrition and Food Chemistry (011001300), Surgery Research Unit (013242220)
- id
- c0191bc8-d2cf-4ff2-ac07-5915eeb8d0f4 (old id 1869174)
- date added to LUP
- 2016-04-01 11:04:21
- date last changed
- 2023-09-14 18:47:23
@article{c0191bc8-d2cf-4ff2-ac07-5915eeb8d0f4, abstract = {{Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved.}}, author = {{Ryberg, Anna and Olsson, Crister and Ahrné, Siv and Monstein, Hans-Juerg}}, issn = {{1872-8359}}, keywords = {{Klebsiella spp.; Molecular typing; (GTG)(5)-PCR; Ribosomal intergenic; transcribed spacer (ITS)-PCR; Fingerprint analysis; Multiple; displacement amplification}}, language = {{eng}}, number = {{2}}, pages = {{183--188}}, publisher = {{Elsevier}}, series = {{Journal of Microbiological Methods}}, title = {{Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates}}, url = {{http://dx.doi.org/10.1016/j.mimet.2010.11.019}}, doi = {{10.1016/j.mimet.2010.11.019}}, volume = {{84}}, year = {{2011}}, }