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Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates

Ryberg, Anna; Olsson, Crister LU ; Ahrné, Siv LU and Monstein, Hans-Juerg (2011) In Journal of Microbiological Methods 84(2). p.183-188
Abstract
Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis... (More)
Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved. (Less)
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Contribution to journal
publication status
published
subject
keywords
Klebsiella spp., Molecular typing, (GTG)(5)-PCR, Ribosomal intergenic, transcribed spacer (ITS)-PCR, Fingerprint analysis, Multiple, displacement amplification
in
Journal of Microbiological Methods
volume
84
issue
2
pages
183 - 188
publisher
Elsevier
external identifiers
  • wos:000287951200005
  • scopus:79251613122
ISSN
1872-8359
DOI
10.1016/j.mimet.2010.11.019
language
English
LU publication?
yes
id
c0191bc8-d2cf-4ff2-ac07-5915eeb8d0f4 (old id 1869174)
date added to LUP
2011-04-04 09:36:51
date last changed
2017-01-01 04:08:27
@article{c0191bc8-d2cf-4ff2-ac07-5915eeb8d0f4,
  abstract     = {Molecular typing of Klebsiella species has become important for monitoring dissemination of beta-lactamase-producers in hospital environments. The present study was designed to evaluate poly-trinucleotide (GTG)(5)- and rDNA intergenic transcribed spacer (ITS)-PCR fingerprint analysis for typing of Klebsiella pneumoniae and Klebsiella oxytoca isolates. Multiple displacement amplified DNA derived from 19K pneumoniae (some with an ESBL-phenotype). 35 K. oxytoca isolates, five K pneumoniae, two K oxytoca, three Raoultella, and one Enterobacter aerogenes type and reference strains underwent (GTG)(5) and ITS-PCR analysis. Dendrograms were constructed using cosine coefficient and the Neighbour joining method. (GTG)(5) and ITS-PCR analysis revealed that K pneumoniae and K oxytoca isolates. reference and type strains formed distinct cluster groups, and tentative subclusters could be established. We conclude that (GTG)(5) and ITS-PCR analysis combined with automated capillary electrophoresis provides promising tools for molecular typing of Klebsiella isolates. (C) 2010 Elsevier B.V. All rights reserved.},
  author       = {Ryberg, Anna and Olsson, Crister and Ahrné, Siv and Monstein, Hans-Juerg},
  issn         = {1872-8359},
  keyword      = {Klebsiella spp.,Molecular typing,(GTG)(5)-PCR,Ribosomal intergenic,transcribed spacer (ITS)-PCR,Fingerprint analysis,Multiple,displacement amplification},
  language     = {eng},
  number       = {2},
  pages        = {183--188},
  publisher    = {Elsevier},
  series       = {Journal of Microbiological Methods},
  title        = {Comparison of (GTG)(5)-oligonucleotide and ribosomal intergenic transcribed spacer (ITS)-PCR for molecular typing of Klebsiella isolates},
  url          = {http://dx.doi.org/10.1016/j.mimet.2010.11.019},
  volume       = {84},
  year         = {2011},
}