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Inclusion of a single-tail amino acid-based amphiphile in a lipoplex formulation: Effects on transfection efficiency and physicochemical properties

Lundberg, Dan LU ; Faneca, Henrique; Moran, Maria Del Carmen; Pedroso De Lima, Maria C.; Miguel, Maria Da Graca and Lindman, Björn LU (2011) In Molecular Membrane Biology 28(1). p.42-53
Abstract
Effects of the addition of a cationic amino acid-based synthetic amphiphile, arginine N-lauroyl amide dihydrochloride (ALA), to a lipid-based transfection formulation have been investigated. It is shown that the inclusion of ALA results in a substantial enhancement of the transfection capability of lipoplexes prepared with liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine and cholesterol, which themselves mediate highly efficient transfection. A possible explanation for the increased biological activity is that ALA adsorbed to the surface of the DNA-lipid complexes is involved in triggering internalization. However, in order to identify possible additional factors underlying the enhanced transfection efficiency, the... (More)
Effects of the addition of a cationic amino acid-based synthetic amphiphile, arginine N-lauroyl amide dihydrochloride (ALA), to a lipid-based transfection formulation have been investigated. It is shown that the inclusion of ALA results in a substantial enhancement of the transfection capability of lipoplexes prepared with liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine and cholesterol, which themselves mediate highly efficient transfection. A possible explanation for the increased biological activity is that ALA adsorbed to the surface of the DNA-lipid complexes is involved in triggering internalization. However, in order to identify possible additional factors underlying the enhanced transfection efficiency, the physical properties of formulations with and without ALA were characterized using cryo-transmission electron microscopy, dynamic light scattering, and an ethidium bromide intercalation assay. ALA seems to have limited influence on the initial internal structure of the complexes and the protection of DNA, but its presence is found to decrease the average effective size of the dispersed particles; this change in size may be important in improving the biological activity. Furthermore, ALA can act to influence the transfection efficiency of the formulation by promoting the release of DNA following internalization in the transfected cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gene therapy, colloid science, mechanism
in
Molecular Membrane Biology
volume
28
issue
1
pages
42 - 53
publisher
Informa Healthcare
external identifiers
  • wos:000287154300004
  • scopus:78651279670
ISSN
0968-7688
DOI
10.3109/09687688.2010.508879
language
English
LU publication?
yes
id
186f74f2-a48c-4e0b-aef9-d7a263bb890c (old id 1877898)
date added to LUP
2011-04-15 15:48:26
date last changed
2017-01-01 06:33:48
@article{186f74f2-a48c-4e0b-aef9-d7a263bb890c,
  abstract     = {Effects of the addition of a cationic amino acid-based synthetic amphiphile, arginine N-lauroyl amide dihydrochloride (ALA), to a lipid-based transfection formulation have been investigated. It is shown that the inclusion of ALA results in a substantial enhancement of the transfection capability of lipoplexes prepared with liposomes of 1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine and cholesterol, which themselves mediate highly efficient transfection. A possible explanation for the increased biological activity is that ALA adsorbed to the surface of the DNA-lipid complexes is involved in triggering internalization. However, in order to identify possible additional factors underlying the enhanced transfection efficiency, the physical properties of formulations with and without ALA were characterized using cryo-transmission electron microscopy, dynamic light scattering, and an ethidium bromide intercalation assay. ALA seems to have limited influence on the initial internal structure of the complexes and the protection of DNA, but its presence is found to decrease the average effective size of the dispersed particles; this change in size may be important in improving the biological activity. Furthermore, ALA can act to influence the transfection efficiency of the formulation by promoting the release of DNA following internalization in the transfected cells.},
  author       = {Lundberg, Dan and Faneca, Henrique and Moran, Maria Del Carmen and Pedroso De Lima, Maria C. and Miguel, Maria Da Graca and Lindman, Björn},
  issn         = {0968-7688},
  keyword      = {Gene therapy,colloid science,mechanism},
  language     = {eng},
  number       = {1},
  pages        = {42--53},
  publisher    = {Informa Healthcare},
  series       = {Molecular Membrane Biology},
  title        = {Inclusion of a single-tail amino acid-based amphiphile in a lipoplex formulation: Effects on transfection efficiency and physicochemical properties},
  url          = {http://dx.doi.org/10.3109/09687688.2010.508879},
  volume       = {28},
  year         = {2011},
}