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Towards a cost-effective immobilized lipase for specialty chemicals

Tufvesson, Pär LU ; Törnvall, Ulrika LU ; Carvalho, Jorge LU ; Karlsson, Annika J. and Hatti-Kaul, Rajni LU (2011) In Journal of Molecular Catalysis B: Enzymatic 68(2). p.200-205
Abstract
Biocatalysis has the potential to provide the chemical industry with several advantages for the production of chemicals; but the use of this technology for the production of speciality and bulk chemicals is unfortunately limited due to the high costs related to the production of the biocatalyst. We have immobilized Candida antarctica lipase B (CALB) on different resins in order to obtain a more cost-effective biocatalyst, i.e. to find the cheapest preparation per catalytic activity, for one esterification and one amidation reaction. It was found that lipase immobilized on macroporous polypropylene (Accurel MP1000) and polymethylmethacrylate (Lewatit) provided biocatalyst preparations where the cost of enzyme and carrier was significantly... (More)
Biocatalysis has the potential to provide the chemical industry with several advantages for the production of chemicals; but the use of this technology for the production of speciality and bulk chemicals is unfortunately limited due to the high costs related to the production of the biocatalyst. We have immobilized Candida antarctica lipase B (CALB) on different resins in order to obtain a more cost-effective biocatalyst, i.e. to find the cheapest preparation per catalytic activity, for one esterification and one amidation reaction. It was found that lipase immobilized on macroporous polypropylene (Accurel MP1000) and polymethylmethacrylate (Lewatit) provided biocatalyst preparations where the cost of enzyme and carrier was significantly less than the cost of commercially available Novozym (R) 435, indicating a potential for decreased cost. Also the stability of Accurel MP1000 bound enzyme during repeated use matched that of Novozym (R) 435. Finally the in-house biocatalyst was used in a packed-bed set-up, showing an excellent stability in repeated batches at 70 degrees C. 2010 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amidation, Esterification, Batch stirred tank reactor, Packed-bed, reactor, Biocatalyst stability
in
Journal of Molecular Catalysis B: Enzymatic
volume
68
issue
2
pages
200 - 205
publisher
Elsevier
external identifiers
  • wos:000286778400012
  • scopus:78650517734
ISSN
1873-3158
DOI
10.1016/j.molcatb.2010.11.004
language
English
LU publication?
yes
id
8cd03b23-8b73-473f-b4ba-058e1bb5cc67 (old id 1877971)
date added to LUP
2011-04-15 15:45:40
date last changed
2017-10-22 03:11:39
@article{8cd03b23-8b73-473f-b4ba-058e1bb5cc67,
  abstract     = {Biocatalysis has the potential to provide the chemical industry with several advantages for the production of chemicals; but the use of this technology for the production of speciality and bulk chemicals is unfortunately limited due to the high costs related to the production of the biocatalyst. We have immobilized Candida antarctica lipase B (CALB) on different resins in order to obtain a more cost-effective biocatalyst, i.e. to find the cheapest preparation per catalytic activity, for one esterification and one amidation reaction. It was found that lipase immobilized on macroporous polypropylene (Accurel MP1000) and polymethylmethacrylate (Lewatit) provided biocatalyst preparations where the cost of enzyme and carrier was significantly less than the cost of commercially available Novozym (R) 435, indicating a potential for decreased cost. Also the stability of Accurel MP1000 bound enzyme during repeated use matched that of Novozym (R) 435. Finally the in-house biocatalyst was used in a packed-bed set-up, showing an excellent stability in repeated batches at 70 degrees C. 2010 Elsevier B.V. All rights reserved.},
  author       = {Tufvesson, Pär and Törnvall, Ulrika and Carvalho, Jorge and Karlsson, Annika J. and Hatti-Kaul, Rajni},
  issn         = {1873-3158},
  keyword      = {Amidation,Esterification,Batch stirred tank reactor,Packed-bed,reactor,Biocatalyst stability},
  language     = {eng},
  number       = {2},
  pages        = {200--205},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Catalysis B: Enzymatic},
  title        = {Towards a cost-effective immobilized lipase for specialty chemicals},
  url          = {http://dx.doi.org/10.1016/j.molcatb.2010.11.004},
  volume       = {68},
  year         = {2011},
}