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DNA-lipid self-assembly: phase behavior and phase structures of a DNA-surfactant complex mixed with lecithin and water

Bilalov, Azat LU ; Olsson, Ulf LU and Lindman, Björn LU (2011) In Soft Matter 7(2). p.730-742
Abstract
The co-assembly of DNA and lipids has broad implications, from the structure of the cell nucleus to gene delivery formulations. To progress in the fundamental understanding of DNA-lipid interactions the self-assembly behavior of a cationic surfactant (dodecyltrimethylammonium, DTA) with DNA as a counterion in mixtures of water and lecithin was investigated. The phase diagram was established and the different regions of the phase diagram characterized with respect to the microstructure by H-2 NMR, small-angle X-ray scattering (SAXS), and other techniques. Three liquid crystalline (LC) phases were identified: cubic, lamellar and hexagonal. All LC phases are stable in the temperature range 20-65 degrees C. The cubic phase is stable only in a... (More)
The co-assembly of DNA and lipids has broad implications, from the structure of the cell nucleus to gene delivery formulations. To progress in the fundamental understanding of DNA-lipid interactions the self-assembly behavior of a cationic surfactant (dodecyltrimethylammonium, DTA) with DNA as a counterion in mixtures of water and lecithin was investigated. The phase diagram was established and the different regions of the phase diagram characterized with respect to the microstructure by H-2 NMR, small-angle X-ray scattering (SAXS), and other techniques. Three liquid crystalline (LC) phases were identified: cubic, lamellar and hexagonal. All LC phases are stable in the temperature range 20-65 degrees C. The cubic phase is stable only in a narrow composition range. Upon increasing the lecithin content the phase transforms to the lamellar phase via an extended biphasic area. Drying of the lamellar phase leads to the formation of hexagonal phase. Adding salt to the ternary mixtures causes a slight increase in the unit cell dimension of the LC phases. Very high ionic strength may lead to DNA release from liquid crystals and the formation of DNA free liquid crystalline phases. In particular we observe a coexistence of two lamellar phases. One contains DNA and one being essentially free of DNA. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
7
issue
2
pages
730 - 742
publisher
Royal Society of Chemistry
external identifiers
  • wos:000286110900052
  • scopus:78651356688
ISSN
1744-6848
DOI
10.1039/c0sm00650e
language
English
LU publication?
yes
id
0a309132-55f1-4eac-ac07-d0630a4d6ab2 (old id 1878220)
date added to LUP
2011-04-07 12:00:47
date last changed
2017-09-24 04:08:19
@article{0a309132-55f1-4eac-ac07-d0630a4d6ab2,
  abstract     = {The co-assembly of DNA and lipids has broad implications, from the structure of the cell nucleus to gene delivery formulations. To progress in the fundamental understanding of DNA-lipid interactions the self-assembly behavior of a cationic surfactant (dodecyltrimethylammonium, DTA) with DNA as a counterion in mixtures of water and lecithin was investigated. The phase diagram was established and the different regions of the phase diagram characterized with respect to the microstructure by H-2 NMR, small-angle X-ray scattering (SAXS), and other techniques. Three liquid crystalline (LC) phases were identified: cubic, lamellar and hexagonal. All LC phases are stable in the temperature range 20-65 degrees C. The cubic phase is stable only in a narrow composition range. Upon increasing the lecithin content the phase transforms to the lamellar phase via an extended biphasic area. Drying of the lamellar phase leads to the formation of hexagonal phase. Adding salt to the ternary mixtures causes a slight increase in the unit cell dimension of the LC phases. Very high ionic strength may lead to DNA release from liquid crystals and the formation of DNA free liquid crystalline phases. In particular we observe a coexistence of two lamellar phases. One contains DNA and one being essentially free of DNA.},
  author       = {Bilalov, Azat and Olsson, Ulf and Lindman, Björn},
  issn         = {1744-6848},
  language     = {eng},
  number       = {2},
  pages        = {730--742},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {DNA-lipid self-assembly: phase behavior and phase structures of a DNA-surfactant complex mixed with lecithin and water},
  url          = {http://dx.doi.org/10.1039/c0sm00650e},
  volume       = {7},
  year         = {2011},
}