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Condensation of DNA using poly(amido amine) dendrimers: effect of salt concentration on aggregate morphology

Carnerup, Anna LU ; Ainalem, Marie-Louise LU ; Alfredsson, Viveka LU and Nylander, Tommy LU (2011) In Soft Matter 7(2). p.760-768
Abstract
The condensation of DNA and poly(amido amine) dendrimers of generation 1, 2, 4, 6, and 8 has been studied as a function of salt concentration in order to reveal the forces that control the aggregate size and morphology. For the lower generation dendrimers (1, 2, and 4) a dramatic increase in aggregate size occurs as a result of an increase in salt concentration. Toroidal aggregates having an outer diameter of up to several hundreds of nm are observed. For the higher generation 6 dendrimers, the size of the condensed DNA aggregates does not change, however, an alteration in morphology is seen at high salt concentration, as more rod-like aggregates are observed. The size and morphology of generation 8 dendrimers are seemingly insensitive to... (More)
The condensation of DNA and poly(amido amine) dendrimers of generation 1, 2, 4, 6, and 8 has been studied as a function of salt concentration in order to reveal the forces that control the aggregate size and morphology. For the lower generation dendrimers (1, 2, and 4) a dramatic increase in aggregate size occurs as a result of an increase in salt concentration. Toroidal aggregates having an outer diameter of up to several hundreds of nm are observed. For the higher generation 6 dendrimers, the size of the condensed DNA aggregates does not change, however, an alteration in morphology is seen at high salt concentration, as more rod-like aggregates are observed. The size and morphology of generation 8 dendrimers are seemingly insensitive to salt concentration. It is believed that the effective neutralisation of the dendrimer and DNA charge in the aggregate is the reason for the observed effects. It is further shown that the 2D hexagonal lattice spacing observed in toroids is close to constant irrespective of the size of the cation responsible for the DNA condensation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
7
issue
2
pages
760 - 768
publisher
Royal Society of Chemistry
external identifiers
  • wos:000286110900055
  • scopus:78651377873
ISSN
1744-6848
DOI
10.1039/c0sm00644k
language
English
LU publication?
yes
id
bf0fa2e2-709f-40b8-89d9-8705bebdfd52 (old id 1878225)
date added to LUP
2011-04-14 09:10:15
date last changed
2017-11-05 04:10:20
@article{bf0fa2e2-709f-40b8-89d9-8705bebdfd52,
  abstract     = {The condensation of DNA and poly(amido amine) dendrimers of generation 1, 2, 4, 6, and 8 has been studied as a function of salt concentration in order to reveal the forces that control the aggregate size and morphology. For the lower generation dendrimers (1, 2, and 4) a dramatic increase in aggregate size occurs as a result of an increase in salt concentration. Toroidal aggregates having an outer diameter of up to several hundreds of nm are observed. For the higher generation 6 dendrimers, the size of the condensed DNA aggregates does not change, however, an alteration in morphology is seen at high salt concentration, as more rod-like aggregates are observed. The size and morphology of generation 8 dendrimers are seemingly insensitive to salt concentration. It is believed that the effective neutralisation of the dendrimer and DNA charge in the aggregate is the reason for the observed effects. It is further shown that the 2D hexagonal lattice spacing observed in toroids is close to constant irrespective of the size of the cation responsible for the DNA condensation.},
  author       = {Carnerup, Anna and Ainalem, Marie-Louise and Alfredsson, Viveka and Nylander, Tommy},
  issn         = {1744-6848},
  language     = {eng},
  number       = {2},
  pages        = {760--768},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {Condensation of DNA using poly(amido amine) dendrimers: effect of salt concentration on aggregate morphology},
  url          = {http://dx.doi.org/10.1039/c0sm00644k},
  volume       = {7},
  year         = {2011},
}