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Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.

Dexlin Mellby, Linda LU ; Sandström Gerdtsson, Anna LU ; Antberg, Linn LU ; Gunnarsson, Jenny; Hansson, Stefan LU ; Borrebaeck, Carl LU and Wingren, Christer LU (2011) In Proteomics 11. p.1550-1554
Abstract
Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Proteomics
volume
11
pages
1550 - 1554
publisher
John Wiley & Sons
external identifiers
  • pmid:21413147
  • wos:000289528800016
  • scopus:79953689582
ISSN
1615-9861
DOI
10.1002/pmic.200900808
project
CREATE Health
language
English
LU publication?
yes
id
5abba12b-ecbf-402e-b68e-6ee2f5a5ddf4 (old id 1883879)
date added to LUP
2011-04-13 15:53:55
date last changed
2017-09-17 04:02:00
@article{5abba12b-ecbf-402e-b68e-6ee2f5a5ddf4,
  abstract     = {Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins.},
  author       = {Dexlin Mellby, Linda and Sandström Gerdtsson, Anna and Antberg, Linn and Gunnarsson, Jenny and Hansson, Stefan and Borrebaeck, Carl and Wingren, Christer},
  issn         = {1615-9861},
  language     = {eng},
  pages        = {1550--1554},
  publisher    = {John Wiley & Sons},
  series       = {Proteomics},
  title        = {Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.},
  url          = {http://dx.doi.org/10.1002/pmic.200900808},
  volume       = {11},
  year         = {2011},
}