Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.
(2011) In Proteomics 11. p.1550-1554- Abstract
- Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1883879
- author
- Dexlin Mellby, Linda LU ; Sandström Gerdtsson, Anna LU ; Antberg, Linn LU ; Gunnarsson, Jenny ; Hansson, Stefan LU ; Borrebaeck, Carl LU and Wingren, Christer LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proteomics
- volume
- 11
- pages
- 1550 - 1554
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:21413147
- wos:000289528800016
- scopus:79953689582
- pmid:21413147
- ISSN
- 1615-9861
- DOI
- 10.1002/pmic.200900808
- language
- English
- LU publication?
- yes
- id
- 5abba12b-ecbf-402e-b68e-6ee2f5a5ddf4 (old id 1883879)
- date added to LUP
- 2016-04-01 10:52:20
- date last changed
- 2022-04-20 06:58:49
@article{5abba12b-ecbf-402e-b68e-6ee2f5a5ddf4, abstract = {{Generating global protein expression profiles, including also membrane proteins, will be crucial for our understanding of biological processes in health and disease. In this study, we have expanded our antibody microarray technology platform and designed the first human recombinant antibody microarray for membrane proteins targeting crude cell lysates and tissue extracts. We have optimized all key technological parameters and successfully developed a setup for extracting, labeling and analyzing non-fractionated membrane proteomes under non-denaturing conditions. Finally, the platform was also extended and shown to be compatible with simultaneous profiling of both membrane proteins and water-soluble proteins.}}, author = {{Dexlin Mellby, Linda and Sandström Gerdtsson, Anna and Antberg, Linn and Gunnarsson, Jenny and Hansson, Stefan and Borrebaeck, Carl and Wingren, Christer}}, issn = {{1615-9861}}, language = {{eng}}, pages = {{1550--1554}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Proteomics}}, title = {{Design of recombinant antibody microarrays for membrane protein profiling of cell lysates and tissue extracts.}}, url = {{http://dx.doi.org/10.1002/pmic.200900808}}, doi = {{10.1002/pmic.200900808}}, volume = {{11}}, year = {{2011}}, }