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Cross-Reactive Protection against Enterohemorrhagic Escherichia coli Infection by Enteropathogenic Escherichia coli in a Mouse Model.

Calderon Toledo, Carla LU ; Arvidsson, Ida LU and Karpman, Diana LU (2011) In Infection and Immunity 79(6). p.2224-2233
Abstract
Enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli are related attaching and effacing (A/E) pathogens. The genes responsible for the A/E pathology are encoded in a chromosomal pathogenicity island termed the locus of enterocyte effacement (LEE). Both pathogens share a high degree of homology in the LEE and additional 'O' islands. EHEC prevalence is much lower in EPEC endemic areas. This may be due to the development of antibodies against common EPEC and EHEC antigens. This study investigated the hypothesis that EPEC infections may protect against EHEC infections. We used a mouse model to inoculate BALB/c mice intragastrically, first with EPEC, followed by EHEC (E. coli O157:H7). Four control groups received either a... (More)
Enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli are related attaching and effacing (A/E) pathogens. The genes responsible for the A/E pathology are encoded in a chromosomal pathogenicity island termed the locus of enterocyte effacement (LEE). Both pathogens share a high degree of homology in the LEE and additional 'O' islands. EHEC prevalence is much lower in EPEC endemic areas. This may be due to the development of antibodies against common EPEC and EHEC antigens. This study investigated the hypothesis that EPEC infections may protect against EHEC infections. We used a mouse model to inoculate BALB/c mice intragastrically, first with EPEC, followed by EHEC (E. coli O157:H7). Four control groups received either a non-pathogenic E. coli (NPEC) strain followed by EHEC (NPEC/EHEC), alternatively PBS/EHEC, EPEC/PBS or PBS/PBS. Mice were monitored for weight loss and symptoms. EPEC colonized the intestine after challenge and mice developed serum antibodies to intimin and E. coli secreted protein B (encoded in the LEE). Prechallenge with an EPEC strain had a protective effect after EHEC infection as few mice developed mild symptoms, from which they recovered. These mice had an increase in body weight similar to control animals and tissue morphology exhibited mild intestinal changes and normal renal histology. All mice that were not pre-challenged with the EPEC strain developed mild to severe symptoms after EHEC infection, weight loss as well as intestinal and renal histopathological changes. These data suggest that EPEC may protect against EHEC infection in this mouse model. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Infection and Immunity
volume
79
issue
6
pages
2224 - 2233
publisher
American Society for Microbiology
external identifiers
  • wos:000290707200009
  • pmid:21402761
  • scopus:79959453127
ISSN
1098-5522
DOI
10.1128/IAI.01024-10
language
English
LU publication?
yes
id
4f6d2aea-e01e-42a7-b998-55e2ca7092a1 (old id 1883958)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21402761?dopt=Abstract
date added to LUP
2011-04-01 15:53:23
date last changed
2017-02-05 03:25:16
@article{4f6d2aea-e01e-42a7-b998-55e2ca7092a1,
  abstract     = {Enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli are related attaching and effacing (A/E) pathogens. The genes responsible for the A/E pathology are encoded in a chromosomal pathogenicity island termed the locus of enterocyte effacement (LEE). Both pathogens share a high degree of homology in the LEE and additional 'O' islands. EHEC prevalence is much lower in EPEC endemic areas. This may be due to the development of antibodies against common EPEC and EHEC antigens. This study investigated the hypothesis that EPEC infections may protect against EHEC infections. We used a mouse model to inoculate BALB/c mice intragastrically, first with EPEC, followed by EHEC (E. coli O157:H7). Four control groups received either a non-pathogenic E. coli (NPEC) strain followed by EHEC (NPEC/EHEC), alternatively PBS/EHEC, EPEC/PBS or PBS/PBS. Mice were monitored for weight loss and symptoms. EPEC colonized the intestine after challenge and mice developed serum antibodies to intimin and E. coli secreted protein B (encoded in the LEE). Prechallenge with an EPEC strain had a protective effect after EHEC infection as few mice developed mild symptoms, from which they recovered. These mice had an increase in body weight similar to control animals and tissue morphology exhibited mild intestinal changes and normal renal histology. All mice that were not pre-challenged with the EPEC strain developed mild to severe symptoms after EHEC infection, weight loss as well as intestinal and renal histopathological changes. These data suggest that EPEC may protect against EHEC infection in this mouse model.},
  author       = {Calderon Toledo, Carla and Arvidsson, Ida and Karpman, Diana},
  issn         = {1098-5522},
  language     = {eng},
  number       = {6},
  pages        = {2224--2233},
  publisher    = {American Society for Microbiology},
  series       = {Infection and Immunity},
  title        = {Cross-Reactive Protection against Enterohemorrhagic Escherichia coli Infection by Enteropathogenic Escherichia coli in a Mouse Model.},
  url          = {http://dx.doi.org/10.1128/IAI.01024-10},
  volume       = {79},
  year         = {2011},
}