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Serum microRNAs in male subfertility—biomarkers and a potential pathogenetic link to metabolic syndrome

Trzybulska, Dorota LU ; Bobjer, Johannes LU ; Giwercman, Aleksander LU and Tsatsanis, Christos LU (2017) In Journal of Assisted Reproduction and Genetics p.1-6
Abstract

Purpose: The purpose of the study was to identify serum microRNAs providing a link between male subfertility and metabolic syndrome (MetS) and validate their diagnostic potential. Methods: Sera were analyzed for fertility and MetS-related parameters in subfertile men (n = 79) and controls (n = 38). Literature review identified miR-155-5p, miR-122-5p, miR-200a-3p, and miR-200c-3p which previously were associated with parameters of fertility as well as metabolic disorders. They were measured in the sera using an absolute quantitation method (qPCR). In order to investigate the value of miRNAs in predicting subfertility, receiver operating characteristic analysis was done. Results: Subfertile men had higher concentrations of miR-155-5p than... (More)

Purpose: The purpose of the study was to identify serum microRNAs providing a link between male subfertility and metabolic syndrome (MetS) and validate their diagnostic potential. Methods: Sera were analyzed for fertility and MetS-related parameters in subfertile men (n = 79) and controls (n = 38). Literature review identified miR-155-5p, miR-122-5p, miR-200a-3p, and miR-200c-3p which previously were associated with parameters of fertility as well as metabolic disorders. They were measured in the sera using an absolute quantitation method (qPCR). In order to investigate the value of miRNAs in predicting subfertility, receiver operating characteristic analysis was done. Results: Subfertile men had higher concentrations of miR-155-5p than controls (p = 0.003) and for miR-200c-3p, the difference was borderline statistically significant (p = 0.05). miR-155-5p and miR-200c-3p were also associated with subfertility in men with no metabolic disturbances (p = 0.008, p = 0.004, respectively). This association was abrogated if any component of MetS was present. The combination of miR-155-5p and miR-200c-3p with follicle-stimulating hormone, being a well-established subfertility parameter, resulted in an overall diagnostic power of AUC = 0.87, which was even higher when men without MetS components were analyzed (AUC = 0.93). Regarding MetS components, statistically significant correlations were found between miR-122-5p and fasting triglycerides, and waist circumference, but no association with subfertility was identified. Conclusions: Among the four miRNAs analyzed, none of them was associated both with male subfertility and MetS components. The ability of miR-155-5p and miR-200c-3p to identify subfertile men was partly overruled by the presence of metabolic disturbances.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Male subfertility, Metabolic disturbances, Serum microRNA
in
Journal of Assisted Reproduction and Genetics
pages
6 pages
publisher
Kluwer
external identifiers
  • scopus:85021761800
ISSN
1058-0468
DOI
10.1007/s10815-017-0989-0
language
English
LU publication?
yes
id
18c7977e-254c-47e0-a6fe-575ab9c548b1
date added to LUP
2017-08-10 11:13:41
date last changed
2017-08-11 03:00:02
@article{18c7977e-254c-47e0-a6fe-575ab9c548b1,
  abstract     = {<p>Purpose: The purpose of the study was to identify serum microRNAs providing a link between male subfertility and metabolic syndrome (MetS) and validate their diagnostic potential. Methods: Sera were analyzed for fertility and MetS-related parameters in subfertile men (n = 79) and controls (n = 38). Literature review identified miR-155-5p, miR-122-5p, miR-200a-3p, and miR-200c-3p which previously were associated with parameters of fertility as well as metabolic disorders. They were measured in the sera using an absolute quantitation method (qPCR). In order to investigate the value of miRNAs in predicting subfertility, receiver operating characteristic analysis was done. Results: Subfertile men had higher concentrations of miR-155-5p than controls (p = 0.003) and for miR-200c-3p, the difference was borderline statistically significant (p = 0.05). miR-155-5p and miR-200c-3p were also associated with subfertility in men with no metabolic disturbances (p = 0.008, p = 0.004, respectively). This association was abrogated if any component of MetS was present. The combination of miR-155-5p and miR-200c-3p with follicle-stimulating hormone, being a well-established subfertility parameter, resulted in an overall diagnostic power of AUC = 0.87, which was even higher when men without MetS components were analyzed (AUC = 0.93). Regarding MetS components, statistically significant correlations were found between miR-122-5p and fasting triglycerides, and waist circumference, but no association with subfertility was identified. Conclusions: Among the four miRNAs analyzed, none of them was associated both with male subfertility and MetS components. The ability of miR-155-5p and miR-200c-3p to identify subfertile men was partly overruled by the presence of metabolic disturbances.</p>},
  author       = {Trzybulska, Dorota and Bobjer, Johannes and Giwercman, Aleksander and Tsatsanis, Christos},
  issn         = {1058-0468},
  keyword      = {Male subfertility,Metabolic disturbances,Serum microRNA},
  language     = {eng},
  month        = {06},
  pages        = {1--6},
  publisher    = {Kluwer},
  series       = {Journal of Assisted Reproduction and Genetics},
  title        = {Serum microRNAs in male subfertility—biomarkers and a potential pathogenetic link to metabolic syndrome},
  url          = {http://dx.doi.org/10.1007/s10815-017-0989-0},
  year         = {2017},
}