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Novel biocatalyst for the asymmetric reduction of ketones : Permeabilized cells of Gluconobacter oxydans

Adlercreutz, Patrick LU (1991) In Enzyme and Microbial Technology 13(1). p.9-14
Abstract

Gluconobacter oxydans (ATCC 621) were permeabilized with toluene and then lyophilized. This crude enzyme preparation was used to reduce eleven ketones to (S)-alcohols with high enantiomeric excess (for most alcohols 93%-99% e.e.). The coenzyme NADH was regenerated either by adding a second enzyme, formate dehydrogenase, and its substrate, formate, or with 2-butanol as a second substrate for the G. oxydans enzyme(s). With the first of these methods, almost complete conversion was achieved. Permeabilized cells immobilized in calcium alginate gel were used for 18 days without any significant loss of catalytic activity.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
alcohol dehydrogenase, asymmetric reduction, Gluconobacter oxydans
in
Enzyme and Microbial Technology
volume
13
issue
1
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:0026085360
ISSN
0141-0229
DOI
10.1016/0141-0229(91)90181-9
language
English
LU publication?
yes
id
18d06ed8-8244-4c33-aed9-7931823cf879
date added to LUP
2019-06-22 18:44:19
date last changed
2020-01-13 02:05:16
@article{18d06ed8-8244-4c33-aed9-7931823cf879,
  abstract     = {<p>Gluconobacter oxydans (ATCC 621) were permeabilized with toluene and then lyophilized. This crude enzyme preparation was used to reduce eleven ketones to (S)-alcohols with high enantiomeric excess (for most alcohols 93%-99% e.e.). The coenzyme NADH was regenerated either by adding a second enzyme, formate dehydrogenase, and its substrate, formate, or with 2-butanol as a second substrate for the G. oxydans enzyme(s). With the first of these methods, almost complete conversion was achieved. Permeabilized cells immobilized in calcium alginate gel were used for 18 days without any significant loss of catalytic activity.</p>},
  author       = {Adlercreutz, Patrick},
  issn         = {0141-0229},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {9--14},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Novel biocatalyst for the asymmetric reduction of ketones : Permeabilized cells of Gluconobacter oxydans},
  url          = {http://dx.doi.org/10.1016/0141-0229(91)90181-9},
  doi          = {10.1016/0141-0229(91)90181-9},
  volume       = {13},
  year         = {1991},
}