Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

DNA movement in sub-20 nm nanoslits

Salieb-Beugelaar, Georgette ; Teapal, Juliane ; van Nieuwkasteele, Jan ; Wijnperlé, Daniël ; Tegenfeldt, Jonas O. LU orcid ; Eijkel, Jan C.T. and van den Berg, Albert (2007) 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 In Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 p.1201-1203
Abstract

The movement of XbaI digested O-DNA in 20 nanometer and O-DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these intermittent movements two different mobilities were calculated, the total averaged mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of... (More)

The movement of XbaI digested O-DNA in 20 nanometer and O-DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these intermittent movements two different mobilities were calculated, the total averaged mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of the buffer was not significant. This work is the first time DNA movement in such shallow constrictions is investigated.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
DNA, Intermittent movement, Nanoslits
host publication
Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
series title
Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
editor
Viovy, Jean-Louis ; Tabeling, Patrick ; Descroix, Stephanie and Malaquin, Laurent
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
conference location
Paris, France
conference dates
2007-10-07 - 2007-10-11
external identifiers
  • scopus:57349152760
ISBN
9780979806407
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2007 CBMS.
id
18f1821b-ed0d-469d-9b5c-09b388b49b9b
date added to LUP
2022-11-10 14:15:57
date last changed
2023-11-12 07:50:18
@inproceedings{18f1821b-ed0d-469d-9b5c-09b388b49b9b,
  abstract     = {{<p>The movement of XbaI digested O-DNA in 20 nanometer and O-DNA in 12 nanometer high slits was investigated. We found that DNA moved intermittently and following preferential pathways, indicating an important influence of surface roughness. From these intermittent movements two different mobilities were calculated, the total averaged mobilities and averaged mobilities between the intermittent sticking events. The friction coefficient per unit length was calculated from the latter mobilities. A three order of magnitude increase was found for the 12 nm slits compared to the theoretical value. The mobility furthermore differs less than one order of magnitude between 20 nm and 12 nm slits, and the influence of varying the ionic strength of the buffer was not significant. This work is the first time DNA movement in such shallow constrictions is investigated.</p>}},
  author       = {{Salieb-Beugelaar, Georgette and Teapal, Juliane and van Nieuwkasteele, Jan and Wijnperlé, Daniël and Tegenfeldt, Jonas O. and Eijkel, Jan C.T. and van den Berg, Albert}},
  booktitle    = {{Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007}},
  editor       = {{Viovy, Jean-Louis and Tabeling, Patrick and Descroix, Stephanie and Malaquin, Laurent}},
  isbn         = {{9780979806407}},
  keywords     = {{DNA; Intermittent movement; Nanoslits}},
  language     = {{eng}},
  pages        = {{1201--1203}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007}},
  title        = {{DNA movement in sub-20 nm nanoslits}},
  year         = {{2007}},
}