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Development of amperometric immunoassays (AIAs) for calpastatin and mu-calpain with possible applications in the biomedical field

Zor, Kinga LU ; Zabara, Alexandru LU ; Gorton, Lo LU and Nistor, Mihaela LU (2011) In Sensors and Actuators B: Chemical 152(2). p.248-253
Abstract
Due to the involvement of calpains and calpastatin in the pathology of several diseases, it is important to create an analytical tool for their accurate and sensitive measurements. In this work, an amperometric immunoassay (AIA) was developed and optimized for quantification of two key proteins from the calpain proteolytic system. The detection is based on the measurement of a steady-state current on screen printed electrodes due to the reduction of the enzymatic product resulting from specific immunoassays, where the measured current is proportional to the concentration of the target analyte. The optimized assays with dynamic ranges from 0.09 to 6 ng/mL for calpastatin and 0.04 to 5 mu g/mL for calpain. offer simplicity, sensitivity and... (More)
Due to the involvement of calpains and calpastatin in the pathology of several diseases, it is important to create an analytical tool for their accurate and sensitive measurements. In this work, an amperometric immunoassay (AIA) was developed and optimized for quantification of two key proteins from the calpain proteolytic system. The detection is based on the measurement of a steady-state current on screen printed electrodes due to the reduction of the enzymatic product resulting from specific immunoassays, where the measured current is proportional to the concentration of the target analyte. The optimized assays with dynamic ranges from 0.09 to 6 ng/mL for calpastatin and 0.04 to 5 mu g/mL for calpain. offer simplicity, sensitivity and possibility for miniaturization. (C) 2011 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amperometric immunoassay, Calpastatin, Calpain, Pathology
in
Sensors and Actuators B: Chemical
volume
152
issue
2
pages
248 - 253
publisher
Elsevier
external identifiers
  • wos:000288877700016
  • scopus:79952442892
ISSN
0925-4005
DOI
10.1016/j.snb.2010.12.016
language
English
LU publication?
yes
id
75d7844d-02aa-4a67-a0d9-30397e272e09 (old id 1918836)
date added to LUP
2011-05-11 14:31:23
date last changed
2017-01-01 06:05:58
@article{75d7844d-02aa-4a67-a0d9-30397e272e09,
  abstract     = {Due to the involvement of calpains and calpastatin in the pathology of several diseases, it is important to create an analytical tool for their accurate and sensitive measurements. In this work, an amperometric immunoassay (AIA) was developed and optimized for quantification of two key proteins from the calpain proteolytic system. The detection is based on the measurement of a steady-state current on screen printed electrodes due to the reduction of the enzymatic product resulting from specific immunoassays, where the measured current is proportional to the concentration of the target analyte. The optimized assays with dynamic ranges from 0.09 to 6 ng/mL for calpastatin and 0.04 to 5 mu g/mL for calpain. offer simplicity, sensitivity and possibility for miniaturization. (C) 2011 Elsevier B.V. All rights reserved.},
  author       = {Zor, Kinga and Zabara, Alexandru and Gorton, Lo and Nistor, Mihaela},
  issn         = {0925-4005},
  keyword      = {Amperometric immunoassay,Calpastatin,Calpain,Pathology},
  language     = {eng},
  number       = {2},
  pages        = {248--253},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Development of amperometric immunoassays (AIAs) for calpastatin and mu-calpain with possible applications in the biomedical field},
  url          = {http://dx.doi.org/10.1016/j.snb.2010.12.016},
  volume       = {152},
  year         = {2011},
}