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Direct Detection Of Single-Nucleotide Polymorphisms In Bacterial DNA By SNPtrap

Gronlund, Hugo; Moen, Birgitte; Hoorfar, Jeffrey; Rådström, Peter LU ; Malorny, Burkhard and Rudi, Knut (2011) In Preparative Biochemistry & Biotechnology 41(2). p.166-174
Abstract
A major challenge with single-nucleotide polymorphism (SNP) fingerprinting of bacteria and higher organisms is the combination of genome-wide screenings with the potential of multiplexing and accurate SNP detection. Single-nucleotide extension by the minisequencing principle represents a technology that both is highly accurate and enables multiplexing. A current bottleneck for direct genome analyses by minisequencing, however, is the sensitivity, since minisequencing relies on linear signal amplification. Here, we present SNPtrap, which is a novel approach that combines the specificity and possibility of multiplexing by minisequencing with the sensitivity obtained by logarithmic signal amplification by polymerase chain reaction (PCR). We... (More)
A major challenge with single-nucleotide polymorphism (SNP) fingerprinting of bacteria and higher organisms is the combination of genome-wide screenings with the potential of multiplexing and accurate SNP detection. Single-nucleotide extension by the minisequencing principle represents a technology that both is highly accurate and enables multiplexing. A current bottleneck for direct genome analyses by minisequencing, however, is the sensitivity, since minisequencing relies on linear signal amplification. Here, we present SNPtrap, which is a novel approach that combines the specificity and possibility of multiplexing by minisequencing with the sensitivity obtained by logarithmic signal amplification by polymerase chain reaction (PCR). We show a SNPtrap proof of principle in a model system for two polymorphic SNP sites in the Salmonella tetrathionate reductase gene (ttrC). (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
multiplex, Salmonella, SNP
in
Preparative Biochemistry & Biotechnology
volume
41
issue
2
pages
166 - 174
publisher
Taylor & Francis
external identifiers
  • wos:000288960600005
  • scopus:79953229607
ISSN
1532-2297
DOI
10.1080/10826068.2011.547366
language
English
LU publication?
yes
id
9e3a534a-3150-4284-bfac-6d9c90eb047a (old id 1918859)
date added to LUP
2011-05-12 15:44:49
date last changed
2017-01-01 05:30:52
@article{9e3a534a-3150-4284-bfac-6d9c90eb047a,
  abstract     = {A major challenge with single-nucleotide polymorphism (SNP) fingerprinting of bacteria and higher organisms is the combination of genome-wide screenings with the potential of multiplexing and accurate SNP detection. Single-nucleotide extension by the minisequencing principle represents a technology that both is highly accurate and enables multiplexing. A current bottleneck for direct genome analyses by minisequencing, however, is the sensitivity, since minisequencing relies on linear signal amplification. Here, we present SNPtrap, which is a novel approach that combines the specificity and possibility of multiplexing by minisequencing with the sensitivity obtained by logarithmic signal amplification by polymerase chain reaction (PCR). We show a SNPtrap proof of principle in a model system for two polymorphic SNP sites in the Salmonella tetrathionate reductase gene (ttrC).},
  author       = {Gronlund, Hugo and Moen, Birgitte and Hoorfar, Jeffrey and Rådström, Peter and Malorny, Burkhard and Rudi, Knut},
  issn         = {1532-2297},
  keyword      = {multiplex,Salmonella,SNP},
  language     = {eng},
  number       = {2},
  pages        = {166--174},
  publisher    = {Taylor & Francis},
  series       = {Preparative Biochemistry & Biotechnology},
  title        = {Direct Detection Of Single-Nucleotide Polymorphisms In Bacterial DNA By SNPtrap},
  url          = {http://dx.doi.org/10.1080/10826068.2011.547366},
  volume       = {41},
  year         = {2011},
}