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Polystyrene replicas of neuronal basal lamina act as excellent guides for regenerating neurites.

Karlsson, Martin LU ; Johansson, Fredrik LU and Kanje, Martin LU (2011) In Acta Biomaterialia 7. p.2910-2918
Abstract
Various scaffolds, natural or artificial, have been used for neural repair, including basal lamina scaffolds obtained through extraction of nerves. Here we tested whether plastic casts of such preparations could be used for neurite guidance. To this end, longitudinal micron thick sections of rat sciatic nerve were extracted with detergents and treated with Dnase, yielding an acellular basal lamina master. From the basal lamina master a polydimethylsiloxane (PDMS) mold was made. Then a polystyrene replica was made using the PDMS mold as the master. The polystyrene replica showed high similarity to the master within nanometer resolution as revealed by scanning electron microscopy. Organ cultured mouse dorsal root ganglia grown on the... (More)
Various scaffolds, natural or artificial, have been used for neural repair, including basal lamina scaffolds obtained through extraction of nerves. Here we tested whether plastic casts of such preparations could be used for neurite guidance. To this end, longitudinal micron thick sections of rat sciatic nerve were extracted with detergents and treated with Dnase, yielding an acellular basal lamina master. From the basal lamina master a polydimethylsiloxane (PDMS) mold was made. Then a polystyrene replica was made using the PDMS mold as the master. The polystyrene replica showed high similarity to the master within nanometer resolution as revealed by scanning electron microscopy. Organ cultured mouse dorsal root ganglia grown on the polystyrene replica and the master preparation exhibited guided outgrowth of neurites as assayed by two-dimensional fast Fourier transform analysis on preparations, where the neurites had been visualized by β-III-tubulin staining. The neurites aligned longitudally in the direction of the original basal lamina tubes. Thus, using inexpensive methods it is possible to make replicas of basal lamina which can be used for neurite guidance. This opens a new avenue for nerve reconstruction. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Axon guidance, Basal lamina, Dorsal root ganglion, Extraction of nerves, Polystyrene
in
Acta Biomaterialia
volume
7
pages
2910 - 2918
publisher
Elsevier
external identifiers
  • wos:000292226000016
  • scopus:79957752139
ISSN
1878-7568
DOI
10.1016/j.actbio.2011.03.029
language
English
LU publication?
yes
id
6c7649d3-323b-49c0-8707-7551477eeda7 (old id 1936800)
date added to LUP
2011-05-16 13:27:14
date last changed
2017-01-01 03:32:29
@article{6c7649d3-323b-49c0-8707-7551477eeda7,
  abstract     = {Various scaffolds, natural or artificial, have been used for neural repair, including basal lamina scaffolds obtained through extraction of nerves. Here we tested whether plastic casts of such preparations could be used for neurite guidance. To this end, longitudinal micron thick sections of rat sciatic nerve were extracted with detergents and treated with Dnase, yielding an acellular basal lamina master. From the basal lamina master a polydimethylsiloxane (PDMS) mold was made. Then a polystyrene replica was made using the PDMS mold as the master. The polystyrene replica showed high similarity to the master within nanometer resolution as revealed by scanning electron microscopy. Organ cultured mouse dorsal root ganglia grown on the polystyrene replica and the master preparation exhibited guided outgrowth of neurites as assayed by two-dimensional fast Fourier transform analysis on preparations, where the neurites had been visualized by β-III-tubulin staining. The neurites aligned longitudally in the direction of the original basal lamina tubes. Thus, using inexpensive methods it is possible to make replicas of basal lamina which can be used for neurite guidance. This opens a new avenue for nerve reconstruction.},
  author       = {Karlsson, Martin and Johansson, Fredrik and Kanje, Martin},
  issn         = {1878-7568},
  keyword      = {Axon guidance,Basal lamina,Dorsal root ganglion,Extraction of nerves,Polystyrene},
  language     = {eng},
  pages        = {2910--2918},
  publisher    = {Elsevier},
  series       = {Acta Biomaterialia},
  title        = {Polystyrene replicas of neuronal basal lamina act as excellent guides for regenerating neurites.},
  url          = {http://dx.doi.org/10.1016/j.actbio.2011.03.029},
  volume       = {7},
  year         = {2011},
}