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The Antiproliferative Effect of Dietary Fiber Phenolic Compounds Ferulic Acid and p-Coumaric Acid on the Cell Cycle of Caco-2 Cells.

Janicke, Birgit; Hegardt, Cecilia LU ; Krogh, Morten; Önning, Gunilla; Åkesson, Björn; Cirenajwis, Helena and Oredsson, Stina (2011) In Nutrition and Cancer 63. p.611-622
Abstract
Epidemiological and animal studies have shown that dietary fiber is protective against the development of colon cancer. Dietary fiber is a rich source of the hydroxycinnamic acids ferulic acid (FA) and p-coumaric acid (p-CA), which both may contribute to the protective effect. We have investigated the effects of FA and p-CA treatment on global gene expression in Caco-2 colon cancer cells. The Caco-2 cells were treated with 150 μM FA or p-CA for 24 h, and gene expression was analyzed with cDNA microarray technique. A total of 517 genes were significantly affected by FA and 901 by p-CA. As we previously have found that FA or p-CA treatment delayed cell cycle progression, we focused on genes involved in proliferation and cell cycle... (More)
Epidemiological and animal studies have shown that dietary fiber is protective against the development of colon cancer. Dietary fiber is a rich source of the hydroxycinnamic acids ferulic acid (FA) and p-coumaric acid (p-CA), which both may contribute to the protective effect. We have investigated the effects of FA and p-CA treatment on global gene expression in Caco-2 colon cancer cells. The Caco-2 cells were treated with 150 μM FA or p-CA for 24 h, and gene expression was analyzed with cDNA microarray technique. A total of 517 genes were significantly affected by FA and 901 by p-CA. As we previously have found that FA or p-CA treatment delayed cell cycle progression, we focused on genes involved in proliferation and cell cycle regulation. The expressions of a number of genes involved in centrosome assembly, such as RABGAP1 and CEP2, were upregulated by FA treatment as well as the gene for the S phase checkpoint protein SMC1L1. p-CA treatment upregulated CDKN1A expression and downregulated CCNA2, CCNB1, MYC, and ODC1. Some proteins corresponding to the affected genes were also studied. Taken together, the changes found can partly explain the effects of FA or p-CA treatment on cell cycle progression, specifically in the S phase by FA and G(2)/M phase by p-CA treatment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nutrition and Cancer
volume
63
pages
611 - 622
publisher
Lawrence Erlbaum Associates
external identifiers
  • wos:000290970100014
  • pmid:21500097
  • scopus:79957606096
ISSN
1532-7914
DOI
10.1080/01635581.2011.538486
language
English
LU publication?
yes
id
9e0c5a30-f914-46af-9a59-4d84c375024e (old id 1936982)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21500097?dopt=Abstract
date added to LUP
2011-05-02 14:48:13
date last changed
2017-10-01 04:58:44
@article{9e0c5a30-f914-46af-9a59-4d84c375024e,
  abstract     = {Epidemiological and animal studies have shown that dietary fiber is protective against the development of colon cancer. Dietary fiber is a rich source of the hydroxycinnamic acids ferulic acid (FA) and p-coumaric acid (p-CA), which both may contribute to the protective effect. We have investigated the effects of FA and p-CA treatment on global gene expression in Caco-2 colon cancer cells. The Caco-2 cells were treated with 150 μM FA or p-CA for 24 h, and gene expression was analyzed with cDNA microarray technique. A total of 517 genes were significantly affected by FA and 901 by p-CA. As we previously have found that FA or p-CA treatment delayed cell cycle progression, we focused on genes involved in proliferation and cell cycle regulation. The expressions of a number of genes involved in centrosome assembly, such as RABGAP1 and CEP2, were upregulated by FA treatment as well as the gene for the S phase checkpoint protein SMC1L1. p-CA treatment upregulated CDKN1A expression and downregulated CCNA2, CCNB1, MYC, and ODC1. Some proteins corresponding to the affected genes were also studied. Taken together, the changes found can partly explain the effects of FA or p-CA treatment on cell cycle progression, specifically in the S phase by FA and G(2)/M phase by p-CA treatment.},
  author       = {Janicke, Birgit and Hegardt, Cecilia and Krogh, Morten and Önning, Gunilla and Åkesson, Björn and Cirenajwis, Helena and Oredsson, Stina},
  issn         = {1532-7914},
  language     = {eng},
  pages        = {611--622},
  publisher    = {Lawrence Erlbaum Associates},
  series       = {Nutrition and Cancer},
  title        = {The Antiproliferative Effect of Dietary Fiber Phenolic Compounds Ferulic Acid and p-Coumaric Acid on the Cell Cycle of Caco-2 Cells.},
  url          = {http://dx.doi.org/10.1080/01635581.2011.538486},
  volume       = {63},
  year         = {2011},
}