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A new murine IgG1 anti-Tn monoclonal antibody with in vivo anti tumour activity.

Welinder, Charlotte LU ; Baldetorp, Bo LU ; Borrebaeck, Carl; Fredlund, Britt-Marie and Jansson, Bo LU (2011) In Glycobiology 21(8). p.1097-1107
Abstract
The Tn antigen (GalNAc alpha-O-Ser/Thr) is heterogeneously synthesised by a variety of tumours and contains an epitope defined by lectins and antibodies as a cluster of alpha GalNAc carbohydrates synthesised within a peptide sequence, which is rich in serine and/or threonine. The Tn antigen has been utilized as a target in vaccine experiments and also used as a biomarker for prognosis of different cancer forms. In this paper we present a new monoclonal antibody, GOD3-2C4, with the clear hallmarks of an anti-Tn antibody. It was generated through somatic cell hybridisation after immunisation of a mouse with a tumour cell line and a Tn carrying mucin. The antibody recognises synthetic Tn antigen and binds breast, colon, lung, ovarian and... (More)
The Tn antigen (GalNAc alpha-O-Ser/Thr) is heterogeneously synthesised by a variety of tumours and contains an epitope defined by lectins and antibodies as a cluster of alpha GalNAc carbohydrates synthesised within a peptide sequence, which is rich in serine and/or threonine. The Tn antigen has been utilized as a target in vaccine experiments and also used as a biomarker for prognosis of different cancer forms. In this paper we present a new monoclonal antibody, GOD3-2C4, with the clear hallmarks of an anti-Tn antibody. It was generated through somatic cell hybridisation after immunisation of a mouse with a tumour cell line and a Tn carrying mucin. The antibody recognises synthetic Tn antigen and binds breast, colon, lung, ovarian and pancreas cancer. The GOD3-2C4 antibody has ADCC activity against Jurkat cells in vitro and for the first time it can be shown that an anti-Tn antibody has a significant in vivo effect on a human cancer cell line grown as a xenograft in SCID mice. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
monoclonal antibody, in vivo therapy, antigen, Tn, anti-carbohydrate, biomarker
in
Glycobiology
volume
21
issue
8
pages
1097 - 1107
publisher
Oxford University Press
external identifiers
  • wos:000292557600012
  • pmid:21470982
  • scopus:79960275033
ISSN
1460-2423
DOI
10.1093/glycob/cwr048
language
English
LU publication?
yes
id
7101089a-2165-40e6-bc7a-e5d5e3a9d4b2 (old id 1937400)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21470982?dopt=Abstract
date added to LUP
2011-05-02 11:12:21
date last changed
2017-07-23 04:26:11
@article{7101089a-2165-40e6-bc7a-e5d5e3a9d4b2,
  abstract     = {The Tn antigen (GalNAc alpha-O-Ser/Thr) is heterogeneously synthesised by a variety of tumours and contains an epitope defined by lectins and antibodies as a cluster of alpha GalNAc carbohydrates synthesised within a peptide sequence, which is rich in serine and/or threonine. The Tn antigen has been utilized as a target in vaccine experiments and also used as a biomarker for prognosis of different cancer forms. In this paper we present a new monoclonal antibody, GOD3-2C4, with the clear hallmarks of an anti-Tn antibody. It was generated through somatic cell hybridisation after immunisation of a mouse with a tumour cell line and a Tn carrying mucin. The antibody recognises synthetic Tn antigen and binds breast, colon, lung, ovarian and pancreas cancer. The GOD3-2C4 antibody has ADCC activity against Jurkat cells in vitro and for the first time it can be shown that an anti-Tn antibody has a significant in vivo effect on a human cancer cell line grown as a xenograft in SCID mice.},
  author       = {Welinder, Charlotte and Baldetorp, Bo and Borrebaeck, Carl and Fredlund, Britt-Marie and Jansson, Bo},
  issn         = {1460-2423},
  keyword      = {monoclonal antibody,in vivo therapy,antigen,Tn,anti-carbohydrate,biomarker},
  language     = {eng},
  number       = {8},
  pages        = {1097--1107},
  publisher    = {Oxford University Press},
  series       = {Glycobiology},
  title        = {A new murine IgG1 anti-Tn monoclonal antibody with in vivo anti tumour activity.},
  url          = {http://dx.doi.org/10.1093/glycob/cwr048},
  volume       = {21},
  year         = {2011},
}