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Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest.

Kucharz, Krzysztof LU ; Wieloch, Tadeusz LU and Toresson, Håkan LU (2011) In Journal of Cerebral Blood Flow and Metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 31. p.1663-1667
Abstract
Neuronal endoplasmic reticulum (ER), continuous from soma to dendritic spines, undergoes rapid fragmentation in response to N-methyl-D-aspartate (NMDA) receptor stimulation in hippocampal slices and neuronal primary cultures. Here, we show that ER fragments in the mouse brain following cardiac arrest (CA) induced brain ischemia. The ER structure was assessed in vivo in cortical pyramidal neurons in transgenic mice expressing ER-targeted GFP using two-photon laser scanning microscopy with fluorescence recovery after photobleaching (FRAP). Endoplasmic reticulum fragmentation occurred 1 to 2 minutes after CA and once induced, fragmentation was rapid (<15 seconds). We propose that acute ER fragmentation may be a protective response against... (More)
Neuronal endoplasmic reticulum (ER), continuous from soma to dendritic spines, undergoes rapid fragmentation in response to N-methyl-D-aspartate (NMDA) receptor stimulation in hippocampal slices and neuronal primary cultures. Here, we show that ER fragments in the mouse brain following cardiac arrest (CA) induced brain ischemia. The ER structure was assessed in vivo in cortical pyramidal neurons in transgenic mice expressing ER-targeted GFP using two-photon laser scanning microscopy with fluorescence recovery after photobleaching (FRAP). Endoplasmic reticulum fragmentation occurred 1 to 2 minutes after CA and once induced, fragmentation was rapid (<15 seconds). We propose that acute ER fragmentation may be a protective response against severe ischemic stress.Journal of Cerebral Blood Flow & Metabolism advance online publication, 6 April 2011; doi:10.1038/jcbfm.2011.37. (Less)
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publication status
published
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in
Journal of Cerebral Blood Flow and Metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
volume
31
pages
1663 - 1667
publisher
Nature Publishing Group
external identifiers
  • wos:000293342100002
  • pmid:21468089
  • scopus:79960982266
ISSN
1559-7016
DOI
10.1038/jcbfm.2011.37
language
English
LU publication?
yes
id
f491c76d-e6d5-4aa0-9c31-778aa09e4c7e (old id 1937483)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21468089?dopt=Abstract
date added to LUP
2011-05-02 10:06:52
date last changed
2017-07-02 04:13:35
@article{f491c76d-e6d5-4aa0-9c31-778aa09e4c7e,
  abstract     = {Neuronal endoplasmic reticulum (ER), continuous from soma to dendritic spines, undergoes rapid fragmentation in response to N-methyl-D-aspartate (NMDA) receptor stimulation in hippocampal slices and neuronal primary cultures. Here, we show that ER fragments in the mouse brain following cardiac arrest (CA) induced brain ischemia. The ER structure was assessed in vivo in cortical pyramidal neurons in transgenic mice expressing ER-targeted GFP using two-photon laser scanning microscopy with fluorescence recovery after photobleaching (FRAP). Endoplasmic reticulum fragmentation occurred 1 to 2 minutes after CA and once induced, fragmentation was rapid (&lt;15 seconds). We propose that acute ER fragmentation may be a protective response against severe ischemic stress.Journal of Cerebral Blood Flow &amp; Metabolism advance online publication, 6 April 2011; doi:10.1038/jcbfm.2011.37.},
  author       = {Kucharz, Krzysztof and Wieloch, Tadeusz and Toresson, Håkan},
  issn         = {1559-7016},
  language     = {eng},
  pages        = {1663--1667},
  publisher    = {Nature Publishing Group},
  series       = {Journal of Cerebral Blood Flow and Metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism},
  title        = {Rapid fragmentation of the endoplasmic reticulum in cortical neurons of the mouse brain in situ following cardiac arrest.},
  url          = {http://dx.doi.org/10.1038/jcbfm.2011.37},
  volume       = {31},
  year         = {2011},
}