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Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.

Faust, Helena LU ; Pastrana, Diana V; Buck, Christopher B; Dillner, Joakim LU and Ekström, Johanna LU (2011) In Journal of Infectious Diseases 203(8). p.1096-1100
Abstract
To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high... (More)
To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high MCV load (P < .01). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
203
issue
8
pages
1096 - 1100
publisher
Oxford University Press
external identifiers
  • wos:000289168500009
  • pmid:21450999
  • scopus:79953320829
ISSN
1537-6613
DOI
10.1093/infdis/jiq173
language
English
LU publication?
yes
id
bd5fad1f-6dc4-4cb0-b421-3f7e9bba54a7 (old id 1937699)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21450999?dopt=Abstract
date added to LUP
2011-05-02 08:34:37
date last changed
2017-07-30 03:58:31
@article{bd5fad1f-6dc4-4cb0-b421-3f7e9bba54a7,
  abstract     = {To validate whether Merkel cell polyomavirus (MCV) serology correlates with MCV infection, we compared real-time polymerase chain reaction results for MCV DNA on fresh-frozen biopsy specimens from various skin lesions and healthy skin from 434 patients to MCV serology results using viruslike particles (VLPs) and MCV neutralization assays. Sixty-five percent of participants were MCV seropositive and 18% were MCV DNA positive. The presence of antibodies was correlated with the presence of virus DNA (odds ratio, 27.85 [95% confidence interval, 6.6-166.5]), with 97% of patients who tested positive for MCV DNA being MCV seropositive. VLP antibody levels correlated to neutralization titers (r = .72), and high antibody levels correlated to high MCV load (P &lt; .01).},
  author       = {Faust, Helena and Pastrana, Diana V and Buck, Christopher B and Dillner, Joakim and Ekström, Johanna},
  issn         = {1537-6613},
  language     = {eng},
  number       = {8},
  pages        = {1096--1100},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {Antibodies to merkel cell polyomavirus correlate to presence of viral DNA in the skin.},
  url          = {http://dx.doi.org/10.1093/infdis/jiq173},
  volume       = {203},
  year         = {2011},
}