Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

The effect of carrier and carrier priming on the kinetics and pattern of somatic mutation in the V chi Ox1 gene

Källberg, Eva LU ; Gray, David and Leanderson, Tomas LU (1995) In European Journal of Immunology 25(8). p.54-2349
Abstract

Priming mice with a chicken gamma globulin (CGG) carrier protein significantly accelerated the onset of somatic mutation in the V chi Ox1 gene when the mice were subsequently immunized with 2-phenyl-5-oxazolone (phOx) coupled to CGG. The first mutations were already detected 7 days after immunization, while in the true primary response, they are not apparent until day 10. It was also found that comparing the mutation pattern of V chi Ox1 genes from hybridomas derived after immunization with phOx coupled to different carriers revealed quite distinct patterns of somatic mutation. Analysis of hybridoma sequences from the primary immune response to phOx-ovalbumin showed that the codons for Ser29, Ser31 and Lys45 were hot-spots for somatic... (More)

Priming mice with a chicken gamma globulin (CGG) carrier protein significantly accelerated the onset of somatic mutation in the V chi Ox1 gene when the mice were subsequently immunized with 2-phenyl-5-oxazolone (phOx) coupled to CGG. The first mutations were already detected 7 days after immunization, while in the true primary response, they are not apparent until day 10. It was also found that comparing the mutation pattern of V chi Ox1 genes from hybridomas derived after immunization with phOx coupled to different carriers revealed quite distinct patterns of somatic mutation. Analysis of hybridoma sequences from the primary immune response to phOx-ovalbumin showed that the codons for Ser29, Ser31 and Lys45 were hot-spots for somatic mutation. Thus, the frequency and pattern of somatic mutations in the V chi Ox1 gene depends on the available T cell help as well as on the complex structure of the immunizing antigen.

(Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Base Sequence, Chickens, Immunoglobulin Variable Region/genetics, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Mutation/genetics, Ovalbumin/therapeutic use, Oxazolone/therapeutic use, Serum Albumin/therapeutic use, gamma-Globulins/therapeutic use
in
European Journal of Immunology
volume
25
issue
8
pages
54 - 2349
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0029101823
  • pmid:7545120
ISSN
0014-2980
DOI
10.1002/eji.1830250834
language
English
LU publication?
yes
id
194771bd-0f0f-4d2b-89ba-2447c42938e8
date added to LUP
2021-10-07 10:46:52
date last changed
2024-01-05 17:46:38
@article{194771bd-0f0f-4d2b-89ba-2447c42938e8,
  abstract     = {{<p>Priming mice with a chicken gamma globulin (CGG) carrier protein significantly accelerated the onset of somatic mutation in the V chi Ox1 gene when the mice were subsequently immunized with 2-phenyl-5-oxazolone (phOx) coupled to CGG. The first mutations were already detected 7 days after immunization, while in the true primary response, they are not apparent until day 10. It was also found that comparing the mutation pattern of V chi Ox1 genes from hybridomas derived after immunization with phOx coupled to different carriers revealed quite distinct patterns of somatic mutation. Analysis of hybridoma sequences from the primary immune response to phOx-ovalbumin showed that the codons for Ser29, Ser31 and Lys45 were hot-spots for somatic mutation. Thus, the frequency and pattern of somatic mutations in the V chi Ox1 gene depends on the available T cell help as well as on the complex structure of the immunizing antigen.</p>}},
  author       = {{Källberg, Eva and Gray, David and Leanderson, Tomas}},
  issn         = {{0014-2980}},
  keywords     = {{Animals; Base Sequence; Chickens; Immunoglobulin Variable Region/genetics; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Mutation/genetics; Ovalbumin/therapeutic use; Oxazolone/therapeutic use; Serum Albumin/therapeutic use; gamma-Globulins/therapeutic use}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{54--2349}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{European Journal of Immunology}},
  title        = {{The effect of carrier and carrier priming on the kinetics and pattern of somatic mutation in the V chi Ox1 gene}},
  url          = {{http://dx.doi.org/10.1002/eji.1830250834}},
  doi          = {{10.1002/eji.1830250834}},
  volume       = {{25}},
  year         = {{1995}},
}