Characterisation of a glycosylated alkyl polyglycoside produced by a cyclodextrin glycosyltransferase by HPLC-ELSD and -MS.
(2011) In Journal of Chromatography. B 879. p.1857-1860- Abstract
- A transglycosylation reaction between an alkyl polyglycoside and α-cyclodextrin catalysed by cyclodextrin glycosyltransferase (CGTase) from Bacillus macerans was investigated. The reaction products were identified by comparison with standards generated by CGTase catalysed modification of pure alkyl glycosides using HPLC-ELSD and -MS analysis. The main products were alkyl glucopyranosides (substrates present in the alkyl polyglycoside) glycosylated with 6 (primary coupling products) or 12 (secondary coupling products) glucose residues. Both α and β anomers were glycosylated.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1971961
- author
- Svensson, David
LU
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography. B
- volume
- 879
- pages
- 1857 - 1860
- publisher
- Elsevier
- external identifiers
-
- wos:000292665600003
- pmid:21621486
- scopus:80955177600
- pmid:21621486
- ISSN
- 1873-376X
- DOI
- 10.1016/j.jchromb.2011.04.037
- language
- English
- LU publication?
- yes
- id
- 0a6fd34b-f63a-439c-bfb2-8204bb1803a9 (old id 1971961)
- date added to LUP
- 2016-04-01 10:45:33
- date last changed
- 2022-01-26 02:15:22
@article{0a6fd34b-f63a-439c-bfb2-8204bb1803a9, abstract = {{A transglycosylation reaction between an alkyl polyglycoside and α-cyclodextrin catalysed by cyclodextrin glycosyltransferase (CGTase) from Bacillus macerans was investigated. The reaction products were identified by comparison with standards generated by CGTase catalysed modification of pure alkyl glycosides using HPLC-ELSD and -MS analysis. The main products were alkyl glucopyranosides (substrates present in the alkyl polyglycoside) glycosylated with 6 (primary coupling products) or 12 (secondary coupling products) glucose residues. Both α and β anomers were glycosylated.}}, author = {{Svensson, David and Adlercreutz, Patrick}}, issn = {{1873-376X}}, language = {{eng}}, pages = {{1857--1860}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Characterisation of a glycosylated alkyl polyglycoside produced by a cyclodextrin glycosyltransferase by HPLC-ELSD and -MS.}}, url = {{http://dx.doi.org/10.1016/j.jchromb.2011.04.037}}, doi = {{10.1016/j.jchromb.2011.04.037}}, volume = {{879}}, year = {{2011}}, }