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Estrogen regulates DNA synthesis in human gingival epithelial cells displaying strong estrogen receptor β immunoreactivity.

Nebel, Daniel LU ; Bratthall, G; Ekblad, Eva LU ; Norderyd, O and Nilsson, Bengt-Olof LU (2011) In Journal of Periodontal Research 46(5). p.622-628
Abstract
Nebel D, Bratthall G, Ekblad E, Norderyd O, Nilsson B-O. Estrogen regulates DNA synthesis in human gingival epithelial cells displaying strong estrogen receptor β immunoreactivity. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01382.x. © 2011 John Wiley & Sons A/S Background and Objective: Estrogen acts via estrogen receptor (ER) α and β. The expression pattern of ERs and their importance in gingival tissues are not fully understood. In this study, we investigate gingival ER expression and effects of estrogen on gingival epithelial cell proliferation. Material and Methods: Gingival biopsies were obtained from both healthy and diseased sites in three male and three female subjects. Expression of ERα and β was determined by... (More)
Nebel D, Bratthall G, Ekblad E, Norderyd O, Nilsson B-O. Estrogen regulates DNA synthesis in human gingival epithelial cells displaying strong estrogen receptor β immunoreactivity. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01382.x. © 2011 John Wiley & Sons A/S Background and Objective: Estrogen acts via estrogen receptor (ER) α and β. The expression pattern of ERs and their importance in gingival tissues are not fully understood. In this study, we investigate gingival ER expression and effects of estrogen on gingival epithelial cell proliferation. Material and Methods: Gingival biopsies were obtained from both healthy and diseased sites in three male and three female subjects. Expression of ERα and β was determined by immunohistochemistry. Effects of 17β-estradiol (E(2) ) on cell proliferation, monitored by measuring DNA synthesis, were studied in cultured human gingival epithelial HGEPp.05 cells. Results: Estrogen receptor β, but not ERα, immunoreactivity was demonstrated in nuclei of epithelial cells in all layers of the gingival epithelium, but also in cells of the lamina propria. No differences were observed between male and female subjects. The same pattern, i.e. high ERβ expression but no ERα expression, was observed in both healthy and diseased sites within each individual. No differences in the intensity of the ERβ immunoreactive signal and the number of ERβ-positive nuclei were observed between healthy and diseased gingiva. Treatment with a physiological concentration of E(2) (10 nm) had no effect on DNA synthesis in ERβ- and ERα-expressing HGEPp.05 cells. In contrast, E(2) at high concentrations (500 nm and 10 μm) reduced DNA synthesis by 60-70%. Conclusion: Human gingival epithelial cells display strong ERβ but low ERα immunoreactivity both in vivo and in culture. Estrogen attenuates gingival epithelial cell DNA synthesis at high but not low concentrations, suggesting a concentration-dependent mechanism. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
estrogen receptor, DNA synthesis, gingival epithelium, immunohistochemistry
in
Journal of Periodontal Research
volume
46
issue
5
pages
622 - 628
publisher
Wiley-Blackwell
external identifiers
  • wos:000294074800015
  • pmid:21615412
  • scopus:80955177486
ISSN
1600-0765
DOI
10.1111/j.1600-0765.2011.01382.x
language
English
LU publication?
yes
id
6dc947f0-a359-4ed6-9796-975b9302bad6 (old id 1972139)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21615412?dopt=Abstract
date added to LUP
2011-06-07 22:33:14
date last changed
2017-07-02 03:02:10
@article{6dc947f0-a359-4ed6-9796-975b9302bad6,
  abstract     = {Nebel D, Bratthall G, Ekblad E, Norderyd O, Nilsson B-O. Estrogen regulates DNA synthesis in human gingival epithelial cells displaying strong estrogen receptor β immunoreactivity. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01382.x. © 2011 John Wiley & Sons A/S Background and Objective: Estrogen acts via estrogen receptor (ER) α and β. The expression pattern of ERs and their importance in gingival tissues are not fully understood. In this study, we investigate gingival ER expression and effects of estrogen on gingival epithelial cell proliferation. Material and Methods: Gingival biopsies were obtained from both healthy and diseased sites in three male and three female subjects. Expression of ERα and β was determined by immunohistochemistry. Effects of 17β-estradiol (E(2) ) on cell proliferation, monitored by measuring DNA synthesis, were studied in cultured human gingival epithelial HGEPp.05 cells. Results: Estrogen receptor β, but not ERα, immunoreactivity was demonstrated in nuclei of epithelial cells in all layers of the gingival epithelium, but also in cells of the lamina propria. No differences were observed between male and female subjects. The same pattern, i.e. high ERβ expression but no ERα expression, was observed in both healthy and diseased sites within each individual. No differences in the intensity of the ERβ immunoreactive signal and the number of ERβ-positive nuclei were observed between healthy and diseased gingiva. Treatment with a physiological concentration of E(2) (10 nm) had no effect on DNA synthesis in ERβ- and ERα-expressing HGEPp.05 cells. In contrast, E(2) at high concentrations (500 nm and 10 μm) reduced DNA synthesis by 60-70%. Conclusion: Human gingival epithelial cells display strong ERβ but low ERα immunoreactivity both in vivo and in culture. Estrogen attenuates gingival epithelial cell DNA synthesis at high but not low concentrations, suggesting a concentration-dependent mechanism.},
  author       = {Nebel, Daniel and Bratthall, G and Ekblad, Eva and Norderyd, O and Nilsson, Bengt-Olof},
  issn         = {1600-0765},
  keyword      = {estrogen receptor,DNA synthesis,gingival epithelium,immunohistochemistry},
  language     = {eng},
  number       = {5},
  pages        = {622--628},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Periodontal Research},
  title        = {Estrogen regulates DNA synthesis in human gingival epithelial cells displaying strong estrogen receptor β immunoreactivity.},
  url          = {http://dx.doi.org/10.1111/j.1600-0765.2011.01382.x},
  volume       = {46},
  year         = {2011},
}