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Leukotriene receptors are differently expressed in fibroblast from peripheral versus central airways in asthmatics and healthy controls.

Tufvesson, Ellen LU ; Nihlberg, Kristian LU ; Westergren-Thorsson, Gunilla LU and Bjermer, Leif LU (2011) In Prostaglandins, Leukotrienes and Essential Fatty Acids 85. p.67-73
Abstract
Leukotrienes are involved in airway inflammation, and are believed to stimulate airway remodeling in asthma. The aim of the project was to investigate the expression of leukotriene receptors in peripheral and central airway fibroblasts. Peripheral and central airway fibroblasts, from asthmatics and healthy controls, were investigated for the amount of cysteinyl-leukotriene receptors (CysLT(1) and CysLT(2)), leukotriene B(4) receptors (BLT(1) and BLT(2)), IL-13 receptor-α(1) (IL-13Rα(1)) and the IL-4 receptor (IL-4R). The mRNA expression of CysLT(1) in fibroblasts from peripheral airways was higher compared to central airways. There was no difference in CysLT(2) between peripheral and central airways. On the contrary, BLT(1) and BLT(2) were... (More)
Leukotrienes are involved in airway inflammation, and are believed to stimulate airway remodeling in asthma. The aim of the project was to investigate the expression of leukotriene receptors in peripheral and central airway fibroblasts. Peripheral and central airway fibroblasts, from asthmatics and healthy controls, were investigated for the amount of cysteinyl-leukotriene receptors (CysLT(1) and CysLT(2)), leukotriene B(4) receptors (BLT(1) and BLT(2)), IL-13 receptor-α(1) (IL-13Rα(1)) and the IL-4 receptor (IL-4R). The mRNA expression of CysLT(1) in fibroblasts from peripheral airways was higher compared to central airways. There was no difference in CysLT(2) between peripheral and central airways. On the contrary, BLT(1) and BLT(2) were lower in fibroblasts from peripheral airways compared to central. The expression of CysLT(1) was higher than CysLT(2) in fibroblasts from peripheral airways, and the expression of BLT(1) was higher than BLT(2) in both peripheral and central airways. Both BLT(1) and BLT(2) were higher in asthmatics compared to healthy controls, while CysLT(1) and CysLT(2) did not differ. The expression of IL-13Rα(1) was higher in asthmatics compared to controls, and correlated to the BLTs. All fibroblasts stained for the different receptor proteins. Leukotriene receptors are differently expressed in fibroblasts from peripheral compared to central airways, which may explain a suggested cysteinyl-leukotriene driven remodeling mainly in the peripheral airways. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
in
Prostaglandins, Leukotrienes and Essential Fatty Acids
volume
85
pages
67 - 73
publisher
Elsevier
external identifiers
  • wos:000293114200003
  • pmid:21596548
  • scopus:79959624262
ISSN
0952-3278
DOI
10.1016/j.plefa.2011.04.025
language
English
LU publication?
yes
id
fc22ed45-ea0d-4f64-97c8-9a962067ee12 (old id 1972302)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21596548?dopt=Abstract
date added to LUP
2011-06-07 21:47:03
date last changed
2017-10-29 04:30:01
@article{fc22ed45-ea0d-4f64-97c8-9a962067ee12,
  abstract     = {Leukotrienes are involved in airway inflammation, and are believed to stimulate airway remodeling in asthma. The aim of the project was to investigate the expression of leukotriene receptors in peripheral and central airway fibroblasts. Peripheral and central airway fibroblasts, from asthmatics and healthy controls, were investigated for the amount of cysteinyl-leukotriene receptors (CysLT(1) and CysLT(2)), leukotriene B(4) receptors (BLT(1) and BLT(2)), IL-13 receptor-α(1) (IL-13Rα(1)) and the IL-4 receptor (IL-4R). The mRNA expression of CysLT(1) in fibroblasts from peripheral airways was higher compared to central airways. There was no difference in CysLT(2) between peripheral and central airways. On the contrary, BLT(1) and BLT(2) were lower in fibroblasts from peripheral airways compared to central. The expression of CysLT(1) was higher than CysLT(2) in fibroblasts from peripheral airways, and the expression of BLT(1) was higher than BLT(2) in both peripheral and central airways. Both BLT(1) and BLT(2) were higher in asthmatics compared to healthy controls, while CysLT(1) and CysLT(2) did not differ. The expression of IL-13Rα(1) was higher in asthmatics compared to controls, and correlated to the BLTs. All fibroblasts stained for the different receptor proteins. Leukotriene receptors are differently expressed in fibroblasts from peripheral compared to central airways, which may explain a suggested cysteinyl-leukotriene driven remodeling mainly in the peripheral airways.},
  author       = {Tufvesson, Ellen and Nihlberg, Kristian and Westergren-Thorsson, Gunilla and Bjermer, Leif},
  issn         = {0952-3278},
  language     = {eng},
  pages        = {67--73},
  publisher    = {Elsevier},
  series       = {Prostaglandins, Leukotrienes and Essential Fatty Acids},
  title        = {Leukotriene receptors are differently expressed in fibroblast from peripheral versus central airways in asthmatics and healthy controls.},
  url          = {http://dx.doi.org/10.1016/j.plefa.2011.04.025},
  volume       = {85},
  year         = {2011},
}