Moraxella catarrhalis outer membrane vesicles carry beta-lactamase and promote survival of Streptococcus pneumoniae and Haemophilus influenzae by inactivating amoxicillin.
Schaar, Viveka LU ; Nordström, Therése LU ; Mörgelin, Matthias LU and Riesbeck, Kristian LU
(2011)
In Antimicrobial Agents and Chemotherapy
55.
p.3845-3853
- Abstract
- Moraxella catarrhalis is a common pathogen found in children with upper respiratory tract infections, and in patients with chronic obstructive pulmonary disease during exacerbations. The bacterial species is often isolated together with Streptococcus pneumoniae and Haemophilus influenzae. Outer membrane vesicles (OMV) are released by M. catarrhalis and contain phospholipids, adhesins, and immunomodulatory compounds such as lipooligosaccharide. We have recently shown that M. catarrhalis OMV exist in patients upon nasopharyngeal colonization. As virtually all M. catarrhalis are β-lactamase positive, the goal of this study was to investigate whether M. catarrhalis OMV carry β-lactamase, and to analyze if OMV consequently can prevent... (More)
- Moraxella catarrhalis is a common pathogen found in children with upper respiratory tract infections, and in patients with chronic obstructive pulmonary disease during exacerbations. The bacterial species is often isolated together with Streptococcus pneumoniae and Haemophilus influenzae. Outer membrane vesicles (OMV) are released by M. catarrhalis and contain phospholipids, adhesins, and immunomodulatory compounds such as lipooligosaccharide. We have recently shown that M. catarrhalis OMV exist in patients upon nasopharyngeal colonization. As virtually all M. catarrhalis are β-lactamase positive, the goal of this study was to investigate whether M. catarrhalis OMV carry β-lactamase, and to analyze if OMV consequently can prevent amoxicillin-induced killing. Recombinant RH4 β-lactamase was produced and antibodies were raised in rabbits. Transmission electron microscopy, flow cytometry and Western blots verified that OMV carried β-lactamase. Moreover, enzyme assays revealed that M. catarrhalis OMV contained active β-lactamase. OMV (25 μg/ml) incubated with amoxicillin for 1 hr completely hydrolyzed amoxicillin at concentrations up to 2.5 μg/ml. In functional experiments, pre-incubation of amoxicillin (10xMIC) with M. catarrhalis OMV fully rescued amoxicillin-susceptible M. catarrhalis, S. pneumoniae and type b or non-typeable H. influenzae from β-lactam-induced killing. Our results suggest that the presence of amoxicillin-resistant M. catarrhalis originating from β-lactamase-containing OMV may pave the way for respiratory pathogens that by definition are susceptible to β-lactam antibiotics. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1972518
- author
- Schaar, Viveka
LU
; Nordström, Therése
LU
; Mörgelin, Matthias
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Antimicrobial Agents and Chemotherapy
- volume
- 55
- pages
- 3845 - 3853
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000292733800026
- pmid:21576428
- scopus:79960339316
- ISSN
- 1098-6596
- DOI
- 10.1128/AAC.01772-10
- language
- English
- LU publication?
- yes
- id
- bb908e72-4b04-4d95-838c-544da68b7878 (old id 1972518)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21576428?dopt=Abstract
- date added to LUP
- 2016-04-01 14:05:43
- date last changed
- 2022-05-19 22:44:18
@article{bb908e72-4b04-4d95-838c-544da68b7878,
abstract = {{Moraxella catarrhalis is a common pathogen found in children with upper respiratory tract infections, and in patients with chronic obstructive pulmonary disease during exacerbations. The bacterial species is often isolated together with Streptococcus pneumoniae and Haemophilus influenzae. Outer membrane vesicles (OMV) are released by M. catarrhalis and contain phospholipids, adhesins, and immunomodulatory compounds such as lipooligosaccharide. We have recently shown that M. catarrhalis OMV exist in patients upon nasopharyngeal colonization. As virtually all M. catarrhalis are β-lactamase positive, the goal of this study was to investigate whether M. catarrhalis OMV carry β-lactamase, and to analyze if OMV consequently can prevent amoxicillin-induced killing. Recombinant RH4 β-lactamase was produced and antibodies were raised in rabbits. Transmission electron microscopy, flow cytometry and Western blots verified that OMV carried β-lactamase. Moreover, enzyme assays revealed that M. catarrhalis OMV contained active β-lactamase. OMV (25 μg/ml) incubated with amoxicillin for 1 hr completely hydrolyzed amoxicillin at concentrations up to 2.5 μg/ml. In functional experiments, pre-incubation of amoxicillin (10xMIC) with M. catarrhalis OMV fully rescued amoxicillin-susceptible M. catarrhalis, S. pneumoniae and type b or non-typeable H. influenzae from β-lactam-induced killing. Our results suggest that the presence of amoxicillin-resistant M. catarrhalis originating from β-lactamase-containing OMV may pave the way for respiratory pathogens that by definition are susceptible to β-lactam antibiotics.}},
author = {{Schaar, Viveka and Nordström, Therése and Mörgelin, Matthias and Riesbeck, Kristian}},
issn = {{1098-6596}},
language = {{eng}},
pages = {{3845--3853}},
publisher = {{American Society for Microbiology}},
series = {{Antimicrobial Agents and Chemotherapy}},
title = {{Moraxella catarrhalis outer membrane vesicles carry beta-lactamase and promote survival of Streptococcus pneumoniae and Haemophilus influenzae by inactivating amoxicillin.}},
url = {{https://lup.lub.lu.se/search/files/3772893/1979647.pdf}},
doi = {{10.1128/AAC.01772-10}},
volume = {{55}},
year = {{2011}},
}