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Noncovalent antibody immobilization on porous silicon combined with miniaturized Solid-Phase Extraction (SPE) for array based immunoMALDI assays

Yan, Hong LU ; Ahmad Tajudin, Asilah LU ; Bengtsson, Martin LU ; Xiao, Shoujun; Laurell, Thomas LU and Ekström, Simon LU (2011) In Analytical Chemistry 83. p.4942-4948
Abstract
This paper presents a new strategy to combine the power of antibody based capturing of target species in complex samples with the benefits of microfluidic reverse phase sample preparation on an integrated sample enrichment target (RP-ISET) and the analysis speed of MALDI MS. The immunoaffinity step is performed on an in-house developed 3D-structured high surface area porous silicon (PSi) matrix, which allows efficient antibody immobilization by surface adsorption without any coupling agents in 30-60 min. The hydrophilic nature of the porous silicon surface at the molecular level displays a low adsorption of background peptides when exposed to complex digests or plasma samples, improving the conditions for the antigen specific extraction... (More)
This paper presents a new strategy to combine the power of antibody based capturing of target species in complex samples with the benefits of microfluidic reverse phase sample preparation on an integrated sample enrichment target (RP-ISET) and the analysis speed of MALDI MS. The immunoaffinity step is performed on an in-house developed 3D-structured high surface area porous silicon (PSi) matrix, which allows efficient antibody immobilization by surface adsorption without any coupling agents in 30-60 min. The hydrophilic nature of the porous silicon surface at the molecular level displays a low adsorption of background peptides when exposed to complex digests or plasma samples, improving the conditions for the antigen specific extraction and subsequent readout. At the same time, the hydrophobic behavior, due to the nanostructured surface, of the PSi material facilitates liquid confinement during the assay. Using a footprint conforming to the standard for 384 well microplates, direct adaption of the protocol into standard sample handling robots is possible. The performance of the proposed immunoaffinity PSi-ISET immunoMALDI (iMALDI) assay was evaluated by specific detection of angiotensin I at a 10 femtomol level in diluted plasma samples (10 μL, 1 nM). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
83
pages
4942 - 4948
publisher
The American Chemical Society
external identifiers
  • wos:000291499800048
  • pmid:21548625
  • scopus:79959241952
ISSN
1520-6882
DOI
10.1021/ac200679t
project
CREATE Health
language
English
LU publication?
yes
id
700b6e91-ed82-4204-860a-0d0b4480dbf7 (old id 1973099)
date added to LUP
2011-06-14 09:45:46
date last changed
2017-09-10 03:03:36
@article{700b6e91-ed82-4204-860a-0d0b4480dbf7,
  abstract     = {This paper presents a new strategy to combine the power of antibody based capturing of target species in complex samples with the benefits of microfluidic reverse phase sample preparation on an integrated sample enrichment target (RP-ISET) and the analysis speed of MALDI MS. The immunoaffinity step is performed on an in-house developed 3D-structured high surface area porous silicon (PSi) matrix, which allows efficient antibody immobilization by surface adsorption without any coupling agents in 30-60 min. The hydrophilic nature of the porous silicon surface at the molecular level displays a low adsorption of background peptides when exposed to complex digests or plasma samples, improving the conditions for the antigen specific extraction and subsequent readout. At the same time, the hydrophobic behavior, due to the nanostructured surface, of the PSi material facilitates liquid confinement during the assay. Using a footprint conforming to the standard for 384 well microplates, direct adaption of the protocol into standard sample handling robots is possible. The performance of the proposed immunoaffinity PSi-ISET immunoMALDI (iMALDI) assay was evaluated by specific detection of angiotensin I at a 10 femtomol level in diluted plasma samples (10 μL, 1 nM).},
  author       = {Yan, Hong and Ahmad Tajudin, Asilah and Bengtsson, Martin and Xiao, Shoujun and Laurell, Thomas and Ekström, Simon},
  issn         = {1520-6882},
  language     = {eng},
  pages        = {4942--4948},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Noncovalent antibody immobilization on porous silicon combined with miniaturized Solid-Phase Extraction (SPE) for array based immunoMALDI assays},
  url          = {http://dx.doi.org/10.1021/ac200679t},
  volume       = {83},
  year         = {2011},
}