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SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse

Carrasco, Cristhian LU ; Baudel, Henrique Macedo; Sendelius, Johan; Modig, Tobias LU ; Roslander, Christian LU ; Galbe, Mats LU ; Hahn-Hägerdal, Bärbel LU ; Zacchi, Guido LU and Lidén, Gunnar LU (2010) In Enzyme and Microbial Technology 46(2). p.64-73
Abstract
Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting

raw material that can be used for fuel ethanol production. In the present study, bagasse was

steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2

as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material

for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57%, with

only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to

hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble... (More)
Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting

raw material that can be used for fuel ethanol production. In the present study, bagasse was

steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2

as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material

for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57%, with

only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to

hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2%,

5% and 8% WIS). The overall highest sugar yield achieved from bagasse was 87% at 2% WIS. Fermentation

tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment,

and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of

Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054.

The pretreatment hydrolysates produced at 2% WIS as well as the enzymatic hydrolysates showed a

complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more

than 60%) was achieved by the strain TMB3400 at 2% WIS. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Sugarcane bagasse Bioethanol Xylose Yeast Saccharomyces cerevisiae
in
Enzyme and Microbial Technology
volume
46
issue
2
pages
64 - 73
publisher
Elsevier
external identifiers
  • wos:000274093600002
  • scopus:71249111390
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2009.10.016
language
English
LU publication?
yes
id
5eb2df6f-c50b-467a-ba74-b24de5969060 (old id 1978093)
date added to LUP
2011-06-21 18:03:33
date last changed
2018-05-29 09:21:16
@article{5eb2df6f-c50b-467a-ba74-b24de5969060,
  abstract     = {Sugarcane bagasse is a lignocellulosic residue obtained from sugarcane milling, and a potentially interesting<br/><br>
raw material that can be used for fuel ethanol production. In the present study, bagasse was<br/><br>
steam pretreated at temperatures between 180 and 205 ◦C, with holding times of 5–10 min using SO2<br/><br>
as a catalyst to determine conditions that provide a good recovery of pentoses and a suitable material<br/><br>
for enzymatic hydrolysis. Pretreatment conducted at 190 ◦C for 5 min gave a pentose yield of 57%, with<br/><br>
only minor amounts of degradation compounds formed. Commercial cellulolytic enzymes were used to<br/><br>
hydrolyze the obtained fiber fractions after pretreatment at different water-insoluble solid contents (2%,<br/><br>
5% and 8% WIS). The overall highest sugar yield achieved from bagasse was 87% at 2% WIS. Fermentation<br/><br>
tests were made on both the pentose-rich hemicellulose hydrolysate obtained from the pretreatment,<br/><br>
and the enzymatic hydrolysates obtained from the fiber fractions using the xylose-fermenting strain of<br/><br>
Saccharomyces cerevisiae TMB3400, as well as the natural xylose-utilizing yeast Pichia stipitis CBS 6054.<br/><br>
The pretreatment hydrolysates produced at 2% WIS as well as the enzymatic hydrolysates showed a<br/><br>
complete glucose fermentability indicating a low toxicity to the yeasts. The best xylose conversion (more<br/><br>
than 60%) was achieved by the strain TMB3400 at 2% WIS.},
  author       = {Carrasco, Cristhian and Baudel, Henrique Macedo and Sendelius, Johan and Modig, Tobias and Roslander, Christian and Galbe, Mats and Hahn-Hägerdal, Bärbel and Zacchi, Guido and Lidén, Gunnar},
  issn         = {0141-0229},
  keyword      = {Sugarcane bagasse
Bioethanol
Xylose
Yeast
Saccharomyces cerevisiae},
  language     = {eng},
  number       = {2},
  pages        = {64--73},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {SO2-catalyzed steam pretreatment and fermentation of enzymatically hydrolyzed sugarcane bagasse},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2009.10.016},
  volume       = {46},
  year         = {2010},
}