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Strategies for the enzymatic enrichment of PUFA from fish oil

Mbatia, Betty LU ; Mattiasson, Bo LU ; Mulaa, Francis and Adlercreutz, Patrick LU (2011) In European Journal of Lipid Science and Technology 113(6). p.717-723
Abstract
PUFA from oil extracted from Nile perch viscera were enriched by selective enzymatic esterification of the free fatty acids (FFA) or by hydrolysis of ethyl esters of the fatty acids from the oil (FA-EE). Quantitative analysis was performed using RP-HPLC coupled to an evaporative light scattering detector (RP-HPLC-ELSD). The lipase from Thermomyces lanuginosus discriminated against docosahexaenoic acid (DHA) most, resulting in the highest DHA/DHA-EE enrichment while lipase from Pseudomonas cepacia discriminated against eicosapentaenoic acid (EPA) most, resulting in the highest EPA/EPA-EE enrichment. The lipases discriminated between DHA and EPA with a higher selectivity when present as ethyl esters (EE) than when in FFA form. Thus when... (More)
PUFA from oil extracted from Nile perch viscera were enriched by selective enzymatic esterification of the free fatty acids (FFA) or by hydrolysis of ethyl esters of the fatty acids from the oil (FA-EE). Quantitative analysis was performed using RP-HPLC coupled to an evaporative light scattering detector (RP-HPLC-ELSD). The lipase from Thermomyces lanuginosus discriminated against docosahexaenoic acid (DHA) most, resulting in the highest DHA/DHA-EE enrichment while lipase from Pseudomonas cepacia discriminated against eicosapentaenoic acid (EPA) most, resulting in the highest EPA/EPA-EE enrichment. The lipases discriminated between DHA and EPA with a higher selectivity when present as ethyl esters (EE) than when in FFA form. Thus when DHA/EPA were enriched to the same level during esterification and hydrolysis reactions, the DHA-EE/EPA-EE recoveries were higher than those of DHA/EPA-FFA. In reactions catalysed by lipase from T. lanuginosus, at 26 mol% DHA/DHA-EE, DHA recovery was 76% while that of DHA-EE was 84%. In reactions catalysed by lipase from P. cepacia, at 11 mol% EPA/EPA-EE, EPA recovery was 79% while that of EPA-EE was 92%. Both esterification of FFA and hydrolysis of FA-EE were more effective for enriching PUFA compared to hydrolysis of the natural oil and are thus attractive process alternatives for the production of products highly enriched in DHA and/or EPA. When there is only one fatty acid residue in each substrate molecule, the full fatty acid selectivity of the lipase can be expressed, which is not the case with triglycerides as substrates. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
DHA, EPA, Esterification, Hydrolysis, Lipase specificity
in
European Journal of Lipid Science and Technology
volume
113
issue
6
pages
717 - 723
publisher
John Wiley & Sons
external identifiers
  • wos:000291562200006
  • scopus:79958847512
ISSN
1438-7697
DOI
10.1002/ejlt.201000560
language
English
LU publication?
yes
id
ee59c45d-2305-492c-98ce-3e0b7e36b82e (old id 1984945)
date added to LUP
2011-07-08 14:59:49
date last changed
2017-10-22 03:17:54
@article{ee59c45d-2305-492c-98ce-3e0b7e36b82e,
  abstract     = {PUFA from oil extracted from Nile perch viscera were enriched by selective enzymatic esterification of the free fatty acids (FFA) or by hydrolysis of ethyl esters of the fatty acids from the oil (FA-EE). Quantitative analysis was performed using RP-HPLC coupled to an evaporative light scattering detector (RP-HPLC-ELSD). The lipase from Thermomyces lanuginosus discriminated against docosahexaenoic acid (DHA) most, resulting in the highest DHA/DHA-EE enrichment while lipase from Pseudomonas cepacia discriminated against eicosapentaenoic acid (EPA) most, resulting in the highest EPA/EPA-EE enrichment. The lipases discriminated between DHA and EPA with a higher selectivity when present as ethyl esters (EE) than when in FFA form. Thus when DHA/EPA were enriched to the same level during esterification and hydrolysis reactions, the DHA-EE/EPA-EE recoveries were higher than those of DHA/EPA-FFA. In reactions catalysed by lipase from T. lanuginosus, at 26 mol% DHA/DHA-EE, DHA recovery was 76% while that of DHA-EE was 84%. In reactions catalysed by lipase from P. cepacia, at 11 mol% EPA/EPA-EE, EPA recovery was 79% while that of EPA-EE was 92%. Both esterification of FFA and hydrolysis of FA-EE were more effective for enriching PUFA compared to hydrolysis of the natural oil and are thus attractive process alternatives for the production of products highly enriched in DHA and/or EPA. When there is only one fatty acid residue in each substrate molecule, the full fatty acid selectivity of the lipase can be expressed, which is not the case with triglycerides as substrates.},
  author       = {Mbatia, Betty and Mattiasson, Bo and Mulaa, Francis and Adlercreutz, Patrick},
  issn         = {1438-7697},
  keyword      = {DHA,EPA,Esterification,Hydrolysis,Lipase specificity},
  language     = {eng},
  number       = {6},
  pages        = {717--723},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Lipid Science and Technology},
  title        = {Strategies for the enzymatic enrichment of PUFA from fish oil},
  url          = {http://dx.doi.org/10.1002/ejlt.201000560},
  volume       = {113},
  year         = {2011},
}