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The glucose/xylose facilitator Gxf1 from Candida intermedia expressed in a xylose-fermenting industrial strain of Saccharomyces cerevisiae increases xylose uptake in SSCF of wheat straw

Fonseca, Cesar; Olofsson, Kim LU ; Ferreira, Carla; Runquist, David LU ; Fonseca, Luis L.; Hahn-Hägerdal, Bärbel LU and Lidén, Gunnar LU (2011) In Enzyme and Microbial Technology 48(6-7). p.518-525
Abstract
Ethanolic fermentation of lignocellulose raw materials requires industrial xylose-fermenting strains capable of complete and efficient D-xylose consumption. A central question in xylose fermentation by Saccharomyces cerevisiae engineered for xylose fermentation is to improve the xylose uptake. In the current study, the glucose/xylose facilitator Gxf1 from Candida intermedia, was expressed in three different xylose-fermenting S. cerevisiae strains of industrial origin. The in vivo effect on aerobic xylose growth and the initial xylose uptake rate were assessed. The expression of Gxf1 resulted in enhanced aerobic xylose growth only for the TMB3400 based strain. It displayed more than a 2-fold higher affinity for D-xylose than the parental... (More)
Ethanolic fermentation of lignocellulose raw materials requires industrial xylose-fermenting strains capable of complete and efficient D-xylose consumption. A central question in xylose fermentation by Saccharomyces cerevisiae engineered for xylose fermentation is to improve the xylose uptake. In the current study, the glucose/xylose facilitator Gxf1 from Candida intermedia, was expressed in three different xylose-fermenting S. cerevisiae strains of industrial origin. The in vivo effect on aerobic xylose growth and the initial xylose uptake rate were assessed. The expression of Gxf1 resulted in enhanced aerobic xylose growth only for the TMB3400 based strain. It displayed more than a 2-fold higher affinity for D-xylose than the parental strain and approximately 2-fold higher initial specific growth rate at 4 g/L D-xylose. Enhanced xylose consumption was furthermore observed when the GXF1-strain was assessed in simultaneous saccharification and co-fermentation (SSCF) of pretreated wheat straw. However, the ethanol yield remained unchanged due to increased by-product formation. Metabolic flux analysis suggested that the expression of the Gxf1 transporter had shifted the control of xylose catabolism from transport to the NAD(+) dependent oxidation of xylitol to xylulose. (C) 2011 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Xylose transport, Industrial Saccharomyces cerevisiae, Simultaneous, saccharification and co-fermentation, Bioethanol, Gxf1
in
Enzyme and Microbial Technology
volume
48
issue
6-7
pages
518 - 525
publisher
Elsevier
external identifiers
  • wos:000291283500013
  • scopus:79955521875
ISSN
0141-0229
DOI
10.1016/j.enzmictec.2011.02.010
language
English
LU publication?
yes
id
1096bba5-fe39-4811-b81b-ba6769580b39 (old id 1985511)
date added to LUP
2011-07-06 13:27:43
date last changed
2017-10-22 03:04:31
@article{1096bba5-fe39-4811-b81b-ba6769580b39,
  abstract     = {Ethanolic fermentation of lignocellulose raw materials requires industrial xylose-fermenting strains capable of complete and efficient D-xylose consumption. A central question in xylose fermentation by Saccharomyces cerevisiae engineered for xylose fermentation is to improve the xylose uptake. In the current study, the glucose/xylose facilitator Gxf1 from Candida intermedia, was expressed in three different xylose-fermenting S. cerevisiae strains of industrial origin. The in vivo effect on aerobic xylose growth and the initial xylose uptake rate were assessed. The expression of Gxf1 resulted in enhanced aerobic xylose growth only for the TMB3400 based strain. It displayed more than a 2-fold higher affinity for D-xylose than the parental strain and approximately 2-fold higher initial specific growth rate at 4 g/L D-xylose. Enhanced xylose consumption was furthermore observed when the GXF1-strain was assessed in simultaneous saccharification and co-fermentation (SSCF) of pretreated wheat straw. However, the ethanol yield remained unchanged due to increased by-product formation. Metabolic flux analysis suggested that the expression of the Gxf1 transporter had shifted the control of xylose catabolism from transport to the NAD(+) dependent oxidation of xylitol to xylulose. (C) 2011 Elsevier Inc. All rights reserved.},
  author       = {Fonseca, Cesar and Olofsson, Kim and Ferreira, Carla and Runquist, David and Fonseca, Luis L. and Hahn-Hägerdal, Bärbel and Lidén, Gunnar},
  issn         = {0141-0229},
  keyword      = {Xylose transport,Industrial Saccharomyces cerevisiae,Simultaneous,saccharification and co-fermentation,Bioethanol,Gxf1},
  language     = {eng},
  number       = {6-7},
  pages        = {518--525},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {The glucose/xylose facilitator Gxf1 from Candida intermedia expressed in a xylose-fermenting industrial strain of Saccharomyces cerevisiae increases xylose uptake in SSCF of wheat straw},
  url          = {http://dx.doi.org/10.1016/j.enzmictec.2011.02.010},
  volume       = {48},
  year         = {2011},
}