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Development of an Electrochemical Meta-Ion Biosensor Using Self-Assembled Peptide Nanofibrils

Bruno, Viguier LU ; Zor, Kinga LU ; Kasotakis, Emmanouil; Mitraki, Anna; Clausen, Casper H.; Svendsen, Winnie E. and Castillo-Leon, Jaime (2011) In ACS Applied Materials and Interfaces 3(5). p.1594-1600
Abstract
This article describes the combination of self-assembled peptide nanofibrils with metal electrodes for the development of an electrochemical metal-ion biosensor. The biological nanofibrils were immobilized on gold electrodes and used as biorecognition elements for the complexation with copper ions. These nanofibrils were obtained under aqueous conditions, at room temperature and outside the clean room. The functionalized gold electrode was evaluated by cyclic voltammetry, impedance spectroscopy, energy dispersive X-ray and atomic force microscopy. The obtained results displayed a layer of nanofibrils able to complex with copper ions in solution. The response of the obtained biosensor was linear up to 50 mu M copper and presented a... (More)
This article describes the combination of self-assembled peptide nanofibrils with metal electrodes for the development of an electrochemical metal-ion biosensor. The biological nanofibrils were immobilized on gold electrodes and used as biorecognition elements for the complexation with copper ions. These nanofibrils were obtained under aqueous conditions, at room temperature and outside the clean room. The functionalized gold electrode was evaluated by cyclic voltammetry, impedance spectroscopy, energy dispersive X-ray and atomic force microscopy. The obtained results displayed a layer of nanofibrils able to complex with copper ions in solution. The response of the obtained biosensor was linear up to 50 mu M copper and presented a sensitivity of 0.68 mu A cm(-2) mu M-1. Moreover, the fabricated sensor could be regenerated to a copper-free state allowing its reutilization. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
self-assembly, peptide, nanofibrils, electrochemical biosensor, copper, biomaterial
in
ACS Applied Materials and Interfaces
volume
3
issue
5
pages
1594 - 1600
publisher
The American Chemical Society
external identifiers
  • wos:000290843800032
  • scopus:80051694801
ISSN
1944-8244
DOI
10.1021/am200149h
language
English
LU publication?
yes
id
551abf39-899f-4a2b-a9a2-080abdce9652 (old id 1986612)
date added to LUP
2011-06-29 11:11:49
date last changed
2017-06-18 03:14:03
@article{551abf39-899f-4a2b-a9a2-080abdce9652,
  abstract     = {This article describes the combination of self-assembled peptide nanofibrils with metal electrodes for the development of an electrochemical metal-ion biosensor. The biological nanofibrils were immobilized on gold electrodes and used as biorecognition elements for the complexation with copper ions. These nanofibrils were obtained under aqueous conditions, at room temperature and outside the clean room. The functionalized gold electrode was evaluated by cyclic voltammetry, impedance spectroscopy, energy dispersive X-ray and atomic force microscopy. The obtained results displayed a layer of nanofibrils able to complex with copper ions in solution. The response of the obtained biosensor was linear up to 50 mu M copper and presented a sensitivity of 0.68 mu A cm(-2) mu M-1. Moreover, the fabricated sensor could be regenerated to a copper-free state allowing its reutilization.},
  author       = {Bruno, Viguier and Zor, Kinga and Kasotakis, Emmanouil and Mitraki, Anna and Clausen, Casper H. and Svendsen, Winnie E. and Castillo-Leon, Jaime},
  issn         = {1944-8244},
  keyword      = {self-assembly,peptide,nanofibrils,electrochemical biosensor,copper,biomaterial},
  language     = {eng},
  number       = {5},
  pages        = {1594--1600},
  publisher    = {The American Chemical Society},
  series       = {ACS Applied Materials and Interfaces},
  title        = {Development of an Electrochemical Meta-Ion Biosensor Using Self-Assembled Peptide Nanofibrils},
  url          = {http://dx.doi.org/10.1021/am200149h},
  volume       = {3},
  year         = {2011},
}