Advanced

Photobleaching-Insensitive Fluorescence Diagnostics in Skin and Brain Tissue

Brydegaard, Mikkel LU ; Haj-Hosseini, N.; Wardell, K. and Andersson-Engels, Stefan LU (2011) In IEEE Photonics Journal 3(3). p.407-421
Abstract
In this paper, we investigate the possibility of using accurate prediction models for the prediction of protoporphyrin bleaching dynamics to achieve photobleaching-insensitive methods to improve the evaluation of data in an existing clinical fluorescence-guided resection technique. To simulate the scenario, measurements were carried out in vivo on skin of healthy volunteers using a compact fiber-based fluorescence spectroscopy system. We have developed an effective method for the parameterization of sequences of bleaching spectra. We analyze convergence and decay rates with respect to initial conditions and excitation irradiance. We also discuss the consequences and the potential for bleaching-insensitive measurements and their... (More)
In this paper, we investigate the possibility of using accurate prediction models for the prediction of protoporphyrin bleaching dynamics to achieve photobleaching-insensitive methods to improve the evaluation of data in an existing clinical fluorescence-guided resection technique. To simulate the scenario, measurements were carried out in vivo on skin of healthy volunteers using a compact fiber-based fluorescence spectroscopy system. We have developed an effective method for the parameterization of sequences of bleaching spectra. We analyze convergence and decay rates with respect to initial conditions and excitation irradiance. We also discuss the consequences and the potential for bleaching-insensitive measurements and their applicability in a few examples from in vivo open brain surgery. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fluorescence-guided resection, FGR, 5-ALA, PpIX, protoporphyrin, photobleaching, laser-induced fluorescence spectroscopy, LIF, optical, diagnostics, tissue optics, skin, brain, dynamic models, system, identification, state space models
in
IEEE Photonics Journal
volume
3
issue
3
pages
407 - 421
publisher
IEEE--Institute of Electrical and Electronics Engineers Inc.
external identifiers
  • wos:000290429400001
  • scopus:79955871701
ISSN
1943-0655
DOI
10.1109/JPHOT.2011.2141656
language
English
LU publication?
yes
id
3ffbaa10-9a9c-450c-bc40-a65ee2f389bd (old id 1987844)
date added to LUP
2011-06-29 08:38:12
date last changed
2017-04-02 03:53:11
@article{3ffbaa10-9a9c-450c-bc40-a65ee2f389bd,
  abstract     = {In this paper, we investigate the possibility of using accurate prediction models for the prediction of protoporphyrin bleaching dynamics to achieve photobleaching-insensitive methods to improve the evaluation of data in an existing clinical fluorescence-guided resection technique. To simulate the scenario, measurements were carried out in vivo on skin of healthy volunteers using a compact fiber-based fluorescence spectroscopy system. We have developed an effective method for the parameterization of sequences of bleaching spectra. We analyze convergence and decay rates with respect to initial conditions and excitation irradiance. We also discuss the consequences and the potential for bleaching-insensitive measurements and their applicability in a few examples from in vivo open brain surgery.},
  author       = {Brydegaard, Mikkel and Haj-Hosseini, N. and Wardell, K. and Andersson-Engels, Stefan},
  issn         = {1943-0655},
  keyword      = {Fluorescence-guided resection,FGR,5-ALA,PpIX,protoporphyrin,photobleaching,laser-induced fluorescence spectroscopy,LIF,optical,diagnostics,tissue optics,skin,brain,dynamic models,system,identification,state space models},
  language     = {eng},
  number       = {3},
  pages        = {407--421},
  publisher    = {IEEE--Institute of Electrical and Electronics Engineers Inc.},
  series       = {IEEE Photonics Journal},
  title        = {Photobleaching-Insensitive Fluorescence Diagnostics in Skin and Brain Tissue},
  url          = {http://dx.doi.org/10.1109/JPHOT.2011.2141656},
  volume       = {3},
  year         = {2011},
}