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Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion

Mizgier, Maria L; Cataldo, Luis R LU ; Gutierrez, Juan; Santos, José L; Casas, Mariana; Llanos, Paola; Contreras-Ferrat, Ariel E; Moro, Cedric; Bouzakri, Karim and Galgani, Jose E (2017) In Journal of Diabetes Research 2017.
Abstract

Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and... (More)

Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and Wistar rat beta cells were incubated for 24 h with control and conditioned media from noninsulin- and insulin-treated myotubes prior to GSIS determination. Conditioned media from insulin-treated versus nontreated myotubes had higher RANTES but lower IL6, IL8, and MCP1 concentration. Qualitative analyses revealed that conditioned media from noninsulin- and insulin-treated myotubes expressed 32 and 23 out of 80 myokines, respectively. Islets incubated with conditioned media from noninsulin-treated myotubes had higher GSIS versus control islets (p< 0.05). Meanwhile, conditioned media from insulin-treated myotubes did not influence GSIS. In beta cells, GSIS was similar across conditions. In conclusion, factors being present in noninsulin-stimulated muscle cell-derived media appear to influence GSIS in mice islets.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Chemokine CCL5, Culture Media, Conditioned, Glucose, Humans, Insulin, Insulin-Secreting Cells, Interleukin-6, Interleukin-8, Islets of Langerhans, Mice, Muscle Fibers, Skeletal, Rats, Rats, Wistar, Journal Article
in
Journal of Diabetes Research
volume
2017
pages
9 pages
publisher
Hindawi Publishing Corporation
external identifiers
  • scopus:85014284166
ISSN
2314-6753
DOI
10.1155/2017/1328573
language
English
LU publication?
no
id
199df4ae-35df-40ab-bd9a-b2dc048e5cbd
date added to LUP
2018-02-22 07:36:24
date last changed
2019-07-02 04:20:59
@article{199df4ae-35df-40ab-bd9a-b2dc048e5cbd,
  abstract     = {<p>Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and Wistar rat beta cells were incubated for 24 h with control and conditioned media from noninsulin- and insulin-treated myotubes prior to GSIS determination. Conditioned media from insulin-treated versus nontreated myotubes had higher RANTES but lower IL6, IL8, and MCP1 concentration. Qualitative analyses revealed that conditioned media from noninsulin- and insulin-treated myotubes expressed 32 and 23 out of 80 myokines, respectively. Islets incubated with conditioned media from noninsulin-treated myotubes had higher GSIS versus control islets (p&lt; 0.05). Meanwhile, conditioned media from insulin-treated myotubes did not influence GSIS. In beta cells, GSIS was similar across conditions. In conclusion, factors being present in noninsulin-stimulated muscle cell-derived media appear to influence GSIS in mice islets.</p>},
  articleno    = {1328573},
  author       = {Mizgier, Maria L and Cataldo, Luis R and Gutierrez, Juan and Santos, José L and Casas, Mariana and Llanos, Paola and Contreras-Ferrat, Ariel E and Moro, Cedric and Bouzakri, Karim and Galgani, Jose E},
  issn         = {2314-6753},
  keyword      = {Animals,Chemokine CCL5,Culture Media, Conditioned,Glucose,Humans,Insulin,Insulin-Secreting Cells,Interleukin-6,Interleukin-8,Islets of Langerhans,Mice,Muscle Fibers, Skeletal,Rats,Rats, Wistar,Journal Article},
  language     = {eng},
  pages        = {9},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Journal of Diabetes Research},
  title        = {Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion},
  url          = {http://dx.doi.org/10.1155/2017/1328573},
  volume       = {2017},
  year         = {2017},
}