Effects of temperature on UV-B-induced DNA damage and photorepair in Arabidopsis thaliana
Li, S S ; Wang, Y and Björn, Lars Olof LU
(2004)
In Journal of Environmental Sciences(China)
16(1).
p.173-176
- Abstract
- DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 64PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 64PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24degreesC was much faster than that at 0degreesC and 12degreesC with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at... (More)
- DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 64PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 64PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24degreesC was much faster than that at 0degreesC and 12degreesC with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12degreesC was very slow as compared with that at 24degreesC and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0degreesC. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/134346
- author
- Li, S S
; Wang, Y
and Björn, Lars Olof
LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Environmental Sciences(China)
- volume
- 16
- issue
- 1
- pages
- 173 - 176
- publisher
- Science China Press
- external identifiers
-
- pmid:14971476
- wos:000188701000036
- scopus:0842334488
- ISSN
- 1001-0742
- project
- Photobiology
- language
- English
- LU publication?
- yes
- id
- 1a0bcabe-5ce5-46d7-a0d9-b7077b622946 (old id 134346)
- alternative location
- http://www.wanfangdata.com.cn/qikan/periodical.Articles/jes-e/jes-2004/0401/040136.htm
- date added to LUP
- 2016-04-01 11:56:15
- date last changed
- 2022-04-28 22:12:10
@article{1a0bcabe-5ce5-46d7-a0d9-b7077b622946,
abstract = {{DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 64PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 64PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24degreesC was much faster than that at 0degreesC and 12degreesC with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12degreesC was very slow as compared with that at 24degreesC and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0degreesC. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications.}},
author = {{Li, S S and Wang, Y and Björn, Lars Olof}},
issn = {{1001-0742}},
language = {{eng}},
number = {{1}},
pages = {{173--176}},
publisher = {{Science China Press}},
series = {{Journal of Environmental Sciences(China)}},
title = {{Effects of temperature on UV-B-induced DNA damage and photorepair in Arabidopsis thaliana}},
url = {{http://www.wanfangdata.com.cn/qikan/periodical.Articles/jes-e/jes-2004/0401/040136.htm}},
volume = {{16}},
year = {{2004}},
}