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Inhibition of colon carcinoma cell migration following treatment with purified venom from lesser weever fish (Trachinus Vipera)

Fezai, Myriam; Slaymi, Chaker; Ben-Attia, Mossadok; Kroemer, Guido; Lang, Florian and Jemaà, Mohamed LU (2017) In Cellular Physiology and Biochemistry 41(6). p.2279-2288
Abstract

Background: Injury by the sting of Lesser weever fish (Trachinus vipera) may lead to severe pain, edema or tissue necrosis. Cellular effects of the venom are still incompletely understood. Previous observations revealed that purified Lesser weever fish venom (LWFV) induces suicidal death of erythrocytes and HCT116 human colon carcinoma cells. The present study addressed the effect of the venom on colon carcinoma cell toxicity, shape and migration both in p53+/+ and/or p53-/- conditions. Methods: Cells were exposed to medium without or with 500 μg/ ml LWFV. Cell shape, cell area and circularity were visualized and quantified by fluorescence microscopy. Cell volume, granularity and cells toxicity were assessed via... (More)

Background: Injury by the sting of Lesser weever fish (Trachinus vipera) may lead to severe pain, edema or tissue necrosis. Cellular effects of the venom are still incompletely understood. Previous observations revealed that purified Lesser weever fish venom (LWFV) induces suicidal death of erythrocytes and HCT116 human colon carcinoma cells. The present study addressed the effect of the venom on colon carcinoma cell toxicity, shape and migration both in p53+/+ and/or p53-/- conditions. Methods: Cells were exposed to medium without or with 500 μg/ ml LWFV. Cell shape, cell area and circularity were visualized and quantified by fluorescence microscopy. Cell volume, granularity and cells toxicity were assessed via the apoptotic parameters dissipation of mitochondrial inner transmembrane potential, phosphatidylserine surface exposure and cell membrane permeabilization were measured utilizing flow cytometry. Cell migration was evaluated using wound healing assay and two-dimensional migration assay. Results: LWFV treatment was followed by a marked change of cell shape and size, significant decrease of cell area and circularity, significant impairment of cell migration, as well as induction of apoptosis after long exposition. Conclusions: LWFV exposure leads to cell shrinkage, increased granularity, apoptosis and impairment of cell migration, effects presumably contributing to LWFV-induced tissue injury.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell volume, Granularity, Lesser weever fish venom, Migration, Mitochondrial pathway of apoptosis, p53
in
Cellular Physiology and Biochemistry
volume
41
issue
6
pages
10 pages
publisher
Karger
external identifiers
  • scopus:85021294058
  • wos:000405728900013
ISSN
1015-8987
DOI
10.1159/000475646
language
English
LU publication?
yes
id
1a32a0d4-b56f-4336-b500-25a00062323e
date added to LUP
2017-07-12 11:07:07
date last changed
2018-01-07 12:11:23
@article{1a32a0d4-b56f-4336-b500-25a00062323e,
  abstract     = {<p>Background: Injury by the sting of Lesser weever fish (Trachinus vipera) may lead to severe pain, edema or tissue necrosis. Cellular effects of the venom are still incompletely understood. Previous observations revealed that purified Lesser weever fish venom (LWFV) induces suicidal death of erythrocytes and HCT116 human colon carcinoma cells. The present study addressed the effect of the venom on colon carcinoma cell toxicity, shape and migration both in p53<sup>+/+</sup> and/or p53<sup>-/-</sup> conditions. Methods: Cells were exposed to medium without or with 500 μg/ ml LWFV. Cell shape, cell area and circularity were visualized and quantified by fluorescence microscopy. Cell volume, granularity and cells toxicity were assessed via the apoptotic parameters dissipation of mitochondrial inner transmembrane potential, phosphatidylserine surface exposure and cell membrane permeabilization were measured utilizing flow cytometry. Cell migration was evaluated using wound healing assay and two-dimensional migration assay. Results: LWFV treatment was followed by a marked change of cell shape and size, significant decrease of cell area and circularity, significant impairment of cell migration, as well as induction of apoptosis after long exposition. Conclusions: LWFV exposure leads to cell shrinkage, increased granularity, apoptosis and impairment of cell migration, effects presumably contributing to LWFV-induced tissue injury.</p>},
  author       = {Fezai, Myriam and Slaymi, Chaker and Ben-Attia, Mossadok and Kroemer, Guido and Lang, Florian and Jemaà, Mohamed},
  issn         = {1015-8987},
  keyword      = {Cell volume,Granularity,Lesser weever fish venom,Migration,Mitochondrial pathway of apoptosis,p53},
  language     = {eng},
  month        = {06},
  number       = {6},
  pages        = {2279--2288},
  publisher    = {Karger},
  series       = {Cellular Physiology and Biochemistry},
  title        = {Inhibition of colon carcinoma cell migration following treatment with purified venom from lesser weever fish (Trachinus Vipera)},
  url          = {http://dx.doi.org/10.1159/000475646},
  volume       = {41},
  year         = {2017},
}