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Heparin-binding protein in sputum as a marker of pulmonary inflammation, lung function, and bacterial load in children with cystic fibrosis

Hovold, Gisela LU ; Palmcrantz, Victoria ; Kahn, Fredrik LU ; Egesten, Arne LU and Påhlman, Lisa I. LU (2018) In BMC Pulmonary Medicine 18(1).
Abstract

Background: Cystic fibros.000000 bacterial pulmonary infections and neutrophil-dominated inflammation in the airways. The aim of this study was to evaluate the neutrophil-derived protein Heparin-binding protein (HBP) as a potential sputum marker of airway inflammation and bacterial load. Methods: Nineteen CF patients, aged 6-18 years, were prospectively followed for 6 months with sputum sampling at every visit to the CF clinic. A total of 41 sputum samples were collected. Sputum-HBP was analysed with ELISA, neutrophil elastase activity with a chromogenic assay, and total bacterial load with RT-PCR of the 16 s rDNA gene. Data were compared to lung function parameters and airway symptoms. Results: HBP and elastase correlated to a decrease... (More)

Background: Cystic fibros.000000 bacterial pulmonary infections and neutrophil-dominated inflammation in the airways. The aim of this study was to evaluate the neutrophil-derived protein Heparin-binding protein (HBP) as a potential sputum marker of airway inflammation and bacterial load. Methods: Nineteen CF patients, aged 6-18 years, were prospectively followed for 6 months with sputum sampling at every visit to the CF clinic. A total of 41 sputum samples were collected. Sputum-HBP was analysed with ELISA, neutrophil elastase activity with a chromogenic assay, and total bacterial load with RT-PCR of the 16 s rDNA gene. Data were compared to lung function parameters and airway symptoms. Results: HBP and elastase correlated to a decrease in FEV1%predicted compared to the patients' individual baseline pulmonary function (ΔFEV1), but not to bacterial load. Area under the receiver operating characteristic curve values for the detection of > 10% decrease in ΔFEV1 were 0.80 for HBP, 0.78 for elastase, and 0.54 for bacterial load. Conclusions: Sputum HBP is a promising marker of airway inflammation and pulmonary function in children with CF.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Airway infection, Children, Cystic fibrosis, Inflammation, Lung function, Sputum
in
BMC Pulmonary Medicine
volume
18
issue
1
article number
104
publisher
BioMed Central (BMC)
external identifiers
  • pmid:29925362
  • scopus:85048869257
ISSN
1471-2466
DOI
10.1186/s12890-018-0668-7
language
English
LU publication?
yes
id
1a358d9d-9114-49cf-8bc6-6d3877a962dc
date added to LUP
2018-07-05 12:44:00
date last changed
2020-10-20 03:57:42
@article{1a358d9d-9114-49cf-8bc6-6d3877a962dc,
  abstract     = {<p>Background: Cystic fibros.000000 bacterial pulmonary infections and neutrophil-dominated inflammation in the airways. The aim of this study was to evaluate the neutrophil-derived protein Heparin-binding protein (HBP) as a potential sputum marker of airway inflammation and bacterial load. Methods: Nineteen CF patients, aged 6-18 years, were prospectively followed for 6 months with sputum sampling at every visit to the CF clinic. A total of 41 sputum samples were collected. Sputum-HBP was analysed with ELISA, neutrophil elastase activity with a chromogenic assay, and total bacterial load with RT-PCR of the 16 s rDNA gene. Data were compared to lung function parameters and airway symptoms. Results: HBP and elastase correlated to a decrease in FEV<sub>1</sub>%predicted compared to the patients' individual baseline pulmonary function (ΔFEV<sub>1</sub>), but not to bacterial load. Area under the receiver operating characteristic curve values for the detection of &gt; 10% decrease in ΔFEV<sub>1</sub> were 0.80 for HBP, 0.78 for elastase, and 0.54 for bacterial load. Conclusions: Sputum HBP is a promising marker of airway inflammation and pulmonary function in children with CF.</p>},
  author       = {Hovold, Gisela and Palmcrantz, Victoria and Kahn, Fredrik and Egesten, Arne and Påhlman, Lisa I.},
  issn         = {1471-2466},
  language     = {eng},
  month        = {06},
  number       = {1},
  publisher    = {BioMed Central (BMC)},
  series       = {BMC Pulmonary Medicine},
  title        = {Heparin-binding protein in sputum as a marker of pulmonary inflammation, lung function, and bacterial load in children with cystic fibrosis},
  url          = {http://dx.doi.org/10.1186/s12890-018-0668-7},
  doi          = {10.1186/s12890-018-0668-7},
  volume       = {18},
  year         = {2018},
}