Spraying enzymes in microemulsions of AOT in nonpolar organic solvents for fabrication of enzyme electrodes
(2005) In Analytical Chemistry 77(21). p.7074-7079- Abstract
- A new technique suitable for automated, large-scale fabrication of enzyme electrodes by air-spraying enzymes in organic inks is presented. Model oxidoreductases, tyrosinase (Tyr) and glucose oxidase (GOx), were adapted to octane-based ink by entrapment in a system of reverse micelles (RM) of surfactant AOT in octane to separate and stabilize the catalytically active forms of the enzymes in nonpolar organic media. Nonpolar caoutchouk polymer was also used to create a kind of "dry micelles" at the electrode/solution interface. Enzyme/RM/polymer-containing organic inks were air-brushed onto conductive supports and were subsequently covered by sprayed Nafion membranes. The air-brushed enzyme electrodes exhibited relevant bioelectrocatalytic... (More)
- A new technique suitable for automated, large-scale fabrication of enzyme electrodes by air-spraying enzymes in organic inks is presented. Model oxidoreductases, tyrosinase (Tyr) and glucose oxidase (GOx), were adapted to octane-based ink by entrapment in a system of reverse micelles (RM) of surfactant AOT in octane to separate and stabilize the catalytically active forms of the enzymes in nonpolar organic media. Nonpolar caoutchouk polymer was also used to create a kind of "dry micelles" at the electrode/solution interface. Enzyme/RM/polymer-containing organic inks were air-brushed onto conductive supports and were subsequently covered by sprayed Nafion membranes. The air-brushed enzyme electrodes exhibited relevant bioelectrocatalytic activity toward catechol and glucose, with a linear detection range of 0.1-100 mu M catechol and 0.5-7 mM glucose; the sensitivities were 2.41 A M-1 cm(-2) and 2.98 mA M-1 cm(-2) for Tyr and GOx electrodes, respectively. The proposed technique of air-brushing enzymes in organic inks enables automated construction of disposable enzyme electrodes of various designs on a mass-production scale. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/150881
- author
- Shipovskov, Stepan LU ; Trofimova, D ; Saprykin, E ; Christenson, Andreas LU ; Ruzgas, Tautgirdas LU ; Levashov, A V and Ferapontova, Elena LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 77
- issue
- 21
- pages
- 7074 - 7079
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000233125400053
- scopus:27544478114
- pmid:16255612
- ISSN
- 1520-6882
- DOI
- 10.1021/ac050505d
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)
- id
- 1a9585d1-de06-4e91-9431-50f1da8cbd50 (old id 150881)
- date added to LUP
- 2016-04-01 11:32:44
- date last changed
- 2022-01-26 06:52:32
@article{1a9585d1-de06-4e91-9431-50f1da8cbd50,
abstract = {{A new technique suitable for automated, large-scale fabrication of enzyme electrodes by air-spraying enzymes in organic inks is presented. Model oxidoreductases, tyrosinase (Tyr) and glucose oxidase (GOx), were adapted to octane-based ink by entrapment in a system of reverse micelles (RM) of surfactant AOT in octane to separate and stabilize the catalytically active forms of the enzymes in nonpolar organic media. Nonpolar caoutchouk polymer was also used to create a kind of "dry micelles" at the electrode/solution interface. Enzyme/RM/polymer-containing organic inks were air-brushed onto conductive supports and were subsequently covered by sprayed Nafion membranes. The air-brushed enzyme electrodes exhibited relevant bioelectrocatalytic activity toward catechol and glucose, with a linear detection range of 0.1-100 mu M catechol and 0.5-7 mM glucose; the sensitivities were 2.41 A M-1 cm(-2) and 2.98 mA M-1 cm(-2) for Tyr and GOx electrodes, respectively. The proposed technique of air-brushing enzymes in organic inks enables automated construction of disposable enzyme electrodes of various designs on a mass-production scale.}},
author = {{Shipovskov, Stepan and Trofimova, D and Saprykin, E and Christenson, Andreas and Ruzgas, Tautgirdas and Levashov, A V and Ferapontova, Elena}},
issn = {{1520-6882}},
language = {{eng}},
number = {{21}},
pages = {{7074--7079}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Spraying enzymes in microemulsions of AOT in nonpolar organic solvents for fabrication of enzyme electrodes}},
url = {{http://dx.doi.org/10.1021/ac050505d}},
doi = {{10.1021/ac050505d}},
volume = {{77}},
year = {{2005}},
}