OPTIMISATION AND PERFORMANCE QUANTIFICATION ACOUSTIC TRAPPING USING SYNTHETIC NANOPARTICLES
(2021) 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 p.903-904- Abstract
Acoustic trapping is becoming increasingly popular as a tool to isolate and enrich biological nanoparticles [1] (particularly extracellular vesicles [2]), yet an inherent problem with acoustic trapping of nanoparticles is calibration of the trapping efficiency. This abstract presents in-house synthesized nanoparticles with different sound scattering properties, either rigid high density gold nanoparticles (AuNPs) as model system for inorganic nanoparticles (NPs) or synthetic lipid bilayer nano vesicles to mimic the acoustic properties of biological vesicles. The rapid NP synthesis facilitates characterisation of the trapping performance of a single node acoustic trapping system, e.g. by designing application specific NPs size ladders.... (More)
Acoustic trapping is becoming increasingly popular as a tool to isolate and enrich biological nanoparticles [1] (particularly extracellular vesicles [2]), yet an inherent problem with acoustic trapping of nanoparticles is calibration of the trapping efficiency. This abstract presents in-house synthesized nanoparticles with different sound scattering properties, either rigid high density gold nanoparticles (AuNPs) as model system for inorganic nanoparticles (NPs) or synthetic lipid bilayer nano vesicles to mimic the acoustic properties of biological vesicles. The rapid NP synthesis facilitates characterisation of the trapping performance of a single node acoustic trapping system, e.g. by designing application specific NPs size ladders. This is shown by the trapping of AuNPs of two different sizes and synthetic lipid bilayer vesicles and demonstrating a 6-11% NP recovery. The work demonstrates the potential of freshly synthesized nanoparticles as a tool for optimisation of acoustic trapping protocols as well as a fast and simple NP trapping performance verification by visual inspection.
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- author
- Havers, Megan LU ; Novotný, Jakub LU ; Gilbert, Jennifer LU ; Laurell, Thomas LU ; Evander, Mikael LU and Lenshof, Andreas LU
- organization
- publishing date
- 2021
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Acoustic Trapping, Nanoparticles, Synthetic Vesicles
- host publication
- MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
- conference location
- Palm Springs, Virtual, United States
- conference dates
- 2021-10-10 - 2021-10-14
- external identifiers
-
- scopus:85136929306
- ISBN
- 9781733419031
- language
- English
- LU publication?
- yes
- id
- 1ad27a87-3c0e-4683-9c96-a3cfa89e1540
- date added to LUP
- 2022-11-08 10:02:00
- date last changed
- 2023-10-23 06:29:03
@inproceedings{1ad27a87-3c0e-4683-9c96-a3cfa89e1540, abstract = {{<p>Acoustic trapping is becoming increasingly popular as a tool to isolate and enrich biological nanoparticles [1] (particularly extracellular vesicles [2]), yet an inherent problem with acoustic trapping of nanoparticles is calibration of the trapping efficiency. This abstract presents in-house synthesized nanoparticles with different sound scattering properties, either rigid high density gold nanoparticles (AuNPs) as model system for inorganic nanoparticles (NPs) or synthetic lipid bilayer nano vesicles to mimic the acoustic properties of biological vesicles. The rapid NP synthesis facilitates characterisation of the trapping performance of a single node acoustic trapping system, e.g. by designing application specific NPs size ladders. This is shown by the trapping of AuNPs of two different sizes and synthetic lipid bilayer vesicles and demonstrating a 6-11% NP recovery. The work demonstrates the potential of freshly synthesized nanoparticles as a tool for optimisation of acoustic trapping protocols as well as a fast and simple NP trapping performance verification by visual inspection.</p>}}, author = {{Havers, Megan and Novotný, Jakub and Gilbert, Jennifer and Laurell, Thomas and Evander, Mikael and Lenshof, Andreas}}, booktitle = {{MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, isbn = {{9781733419031}}, keywords = {{Acoustic Trapping; Nanoparticles; Synthetic Vesicles}}, language = {{eng}}, pages = {{903--904}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{OPTIMISATION AND PERFORMANCE QUANTIFICATION ACOUSTIC TRAPPING USING SYNTHETIC NANOPARTICLES}}, year = {{2021}}, }