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Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis

Regnault, C.; Punyani, K. LU ; Holm, S. LU ; Beech, J. P. LU ; Otto, Marcia C. de Oliveira; Herold, Kevan C; Herbig, M.; Guck, J.; Tegenfeldt, J. O. LU and Barrett, Michael P (2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.244-245
Abstract

Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Deformability, Diagnosis, Leishmaniasis, Sorting
in
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
external identifiers
  • scopus:85014166507
ISBN
9780979806490
language
English
LU publication?
yes
id
1baebc20-7549-416c-a893-3ff713d8aca9
date added to LUP
2017-03-16 10:29:21
date last changed
2017-03-20 09:48:09
@inproceedings{1baebc20-7549-416c-a893-3ff713d8aca9,
  abstract     = {<p>Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.</p>},
  author       = {Regnault, C. and Punyani, K. and Holm, S. and Beech, J. P. and Otto, Marcia C. de Oliveira and Herold, Kevan C and Herbig, M. and Guck, J. and Tegenfeldt, J. O. and Barrett, Michael P},
  booktitle    = {20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016},
  isbn         = {9780979806490},
  keyword      = {Deformability,Diagnosis,Leishmaniasis,Sorting},
  language     = {eng},
  pages        = {244--245},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis},
  year         = {2016},
}