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Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis

Regnault, C. ; Punyani, K. LU ; Holm, S. LU ; Beech, J. P. LU ; Otto, Marcia C. de Oliveira ; Herold, Kevan C ; Herbig, M. ; Guck, J. ; Tegenfeldt, J. O. LU orcid and Barrett, Michael P (2016) 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p.244-245
Abstract

Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Deformability, Diagnosis, Leishmaniasis, Sorting
host publication
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
conference location
Dublin, Ireland
conference dates
2016-10-09 - 2016-10-13
external identifiers
  • scopus:85014166507
ISBN
9780979806490
language
English
LU publication?
yes
id
1baebc20-7549-416c-a893-3ff713d8aca9
date added to LUP
2017-03-16 10:29:21
date last changed
2023-10-05 01:47:50
@inproceedings{1baebc20-7549-416c-a893-3ff713d8aca9,
  abstract     = {{<p>Microfluidic techniques were applied to the separation of parasite and parasite-infected cells from blood to facilitate the detection of leishmaniasis, a disease representing a high burden in the developing world and for which new diagnostic tools are urgently needed. Leishmania mexicana promastigotes were successfully separated from red blood cells in a deterministic lateral displacement device. The mechanical properties of macrophages infected with L. mexicana were investigated using real-time deformability cytometry. In the early stage, we find that macrophages deform less than the control. The trend is reversed four days post infection while we see a continuous increase in cell size after parasitization.</p>}},
  author       = {{Regnault, C. and Punyani, K. and Holm, S. and Beech, J. P. and Otto, Marcia C. de Oliveira and Herold, Kevan C and Herbig, M. and Guck, J. and Tegenfeldt, J. O. and Barrett, Michael P}},
  booktitle    = {{20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016}},
  isbn         = {{9780979806490}},
  keywords     = {{Deformability; Diagnosis; Leishmaniasis; Sorting}},
  language     = {{eng}},
  pages        = {{244--245}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  title        = {{Microfluidic separation of parasites and parasite-infected cells from blood for the diagnosis of leishmaniasis}},
  year         = {{2016}},
}