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Culture in embryonic kidney serum and xeno-free media as renal cell carcinoma and renal cell carcinoma cancer stem cells research model

Krawczyk, Krzysztof M. LU ; Matak, Damian; Szymanski, Lukasz; Szczylik, Cezary; Porta, Camillo and Czarnecka, Anna M. (2018) In Cytotechnology
Abstract

The use of fetal bovine serum hinders obtaining reproducible experimental results and should also be removed in hormone and growth factor studies. In particular hormones found in FBS act globally on cancer cell physiology and influence transcriptome and metabolome. The aim of our study was to develop a renal carcinoma serum free culture model optimized for (embryonal) renal cells in order to select the best study model for downstream auto-, para- or endocrine research. Secondary aim was to verify renal carcinoma stem cell culture for this application. In the study, we have cultured renal cell carcinoma primary tumour cell line (786-0) as well as human kidney cancer stem cells in standard 2D monolayer cultures in Roswell Park Memorial... (More)

The use of fetal bovine serum hinders obtaining reproducible experimental results and should also be removed in hormone and growth factor studies. In particular hormones found in FBS act globally on cancer cell physiology and influence transcriptome and metabolome. The aim of our study was to develop a renal carcinoma serum free culture model optimized for (embryonal) renal cells in order to select the best study model for downstream auto-, para- or endocrine research. Secondary aim was to verify renal carcinoma stem cell culture for this application. In the study, we have cultured renal cell carcinoma primary tumour cell line (786-0) as well as human kidney cancer stem cells in standard 2D monolayer cultures in Roswell Park Memorial Institute Medium or Dulbecco’s Modified Eagle’s Medium and Complete Human Kidney Cancer Stem Cell Medium, respectively. Serum-free, animal-component free Human Embryonic Kidney 293 media were tested. Our results revealed that xeno-free embryonal renal cells optimized culture media provide a useful tool in RCC cancer biology research and at the same time enable effective growth of RCC. We propose bio-mimic RCC cell culture model with specific serum-free and xeno-free medium that promote RCC cell viability.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Cancer stem cells, Renal cell cancer, Serum-free culture, Xeno-free culture
in
Cytotechnology
pages
22 pages
publisher
Springer
external identifiers
  • scopus:85042071857
ISSN
0920-9069
DOI
10.1007/s10616-017-0181-5
language
English
LU publication?
yes
id
1c08d2fb-0340-4dba-a43b-f6ed0c9c98f9
date added to LUP
2018-03-06 08:43:20
date last changed
2018-06-06 03:00:11
@article{1c08d2fb-0340-4dba-a43b-f6ed0c9c98f9,
  abstract     = {<p>The use of fetal bovine serum hinders obtaining reproducible experimental results and should also be removed in hormone and growth factor studies. In particular hormones found in FBS act globally on cancer cell physiology and influence transcriptome and metabolome. The aim of our study was to develop a renal carcinoma serum free culture model optimized for (embryonal) renal cells in order to select the best study model for downstream auto-, para- or endocrine research. Secondary aim was to verify renal carcinoma stem cell culture for this application. In the study, we have cultured renal cell carcinoma primary tumour cell line (786-0) as well as human kidney cancer stem cells in standard 2D monolayer cultures in Roswell Park Memorial Institute Medium or Dulbecco’s Modified Eagle’s Medium and Complete Human Kidney Cancer Stem Cell Medium, respectively. Serum-free, animal-component free Human Embryonic Kidney 293 media were tested. Our results revealed that xeno-free embryonal renal cells optimized culture media provide a useful tool in RCC cancer biology research and at the same time enable effective growth of RCC. We propose bio-mimic RCC cell culture model with specific serum-free and xeno-free medium that promote RCC cell viability.</p>},
  author       = {Krawczyk, Krzysztof M. and Matak, Damian and Szymanski, Lukasz and Szczylik, Cezary and Porta, Camillo and Czarnecka, Anna M.},
  issn         = {0920-9069},
  keyword      = {Cancer stem cells,Renal cell cancer,Serum-free culture,Xeno-free culture},
  language     = {eng},
  month        = {02},
  pages        = {22},
  publisher    = {Springer},
  series       = {Cytotechnology},
  title        = {Culture in embryonic kidney serum and xeno-free media as renal cell carcinoma and renal cell carcinoma cancer stem cells research model},
  url          = {http://dx.doi.org/10.1007/s10616-017-0181-5},
  year         = {2018},
}